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Titolo:
INFLUENCE OF MOBILE-PHASE CONDITIONS ON THE CLEANUP EFFECT OF RESTRICTED-ACCESS MEDIA PRECOLUMNS FOR PLASMA SAMPLES INJECTED IN A COLUMN-SWITCHING SYSTEM
Autore:
YU Z; WESTERLUND D;
Indirizzi:
UNIV UPPSALA,CTR BIOMED,POB 574 S-75123 UPPSALA SWEDEN
Titolo Testata:
Chromatographia
fascicolo: 11-12, volume: 44, anno: 1997,
pagine: 589 - 594
SICI:
0009-5893(1997)44:11-12<589:IOMCOT>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERFORMANCE LIQUID-CHROMATOGRAPHY; DIRECT SERUM INJECTION; ALKYL-DIOL SILICA; PROTEIN-BINDING; SEMIPERMEABLE SURFACE; RETENTION; DRUGS; PACKING; SELECTIVITY; PHENYTOIN;
Keywords:
COLUMN LIQUID CHROMATOGRAPHY; COLUMN-SWITCHING SYSTEMS; RESTRICTED-ACCESS MEDIA PRECOLUMNS; MOBILE PHASE COMPOSITION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
32
Recensione:
Indirizzi per estratti:
Citazione:
Z. Yu e D. Westerlund, "INFLUENCE OF MOBILE-PHASE CONDITIONS ON THE CLEANUP EFFECT OF RESTRICTED-ACCESS MEDIA PRECOLUMNS FOR PLASMA SAMPLES INJECTED IN A COLUMN-SWITCHING SYSTEM", Chromatographia, 44(11-12), 1997, pp. 589-594

Abstract

Various mobile phases including phosphate buffer, pure water and fivekinds of biological buffers (pH around 7) were systematically studiedin terms of their ability to clean-up plasma matrix on precolumns of restricted-access media in a column-switching system. The necessary washing time, buffer pH, type and content of organic modifier were evaluated with respect to plasma elution profiles on restricted-access media precolumns. The influence of different mobile phases on the recoveryof plasma matrix from alkyl-diol silica precolumns was studied by means of a scanning spectrophotometer. Our results show that phosphate buffer near physiological pH with small amounts of 2-propanol or acetonitrile was preferable for direct injection of large plasma volumes (500mu L). More than 93 % of the proteins in a plasma matrix can be recovered within 3 min from the alkyl-diol silica C-18 column (25 m x 4 mm I.D.) as measured at 280 nm for all selected mobile phases except for tris (hydroxymethyl) aminomethane buffer, from which only about 88 % was obtained.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 14:27:42