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Titolo:
Iron transport into Mycobacterium avium-containing phagosomes from an Nramp1(Gly169)-transfected RAW264.7 macrophage cell line
Autore:
Kuhn, DE; Lafuse, WP; Zwilling, BS;
Indirizzi:
Ohio State Univ, Dept Microbiol, Columbus, OH 43210 USA Ohio State Univ Columbus OH USA 43210 t Microbiol, Columbus, OH 43210 USA Ohio State Univ, Dept Mol Virol, Columbus, OH 43210 USA Ohio State Univ Columbus OH USA 43210 t Mol Virol, Columbus, OH 43210 USA Ohio State Univ, Dept Immunol, Columbus, OH 43210 USA Ohio State Univ Columbus OH USA 43210 ept Immunol, Columbus, OH 43210 USA Ohio State Univ, Dept Med Genet, Columbus, OH 43210 USA Ohio State Univ Columbus OH USA 43210 t Med Genet, Columbus, OH 43210 USA
Titolo Testata:
JOURNAL OF LEUKOCYTE BIOLOGY
fascicolo: 1, volume: 69, anno: 2001,
pagine: 43 - 49
SICI:
0741-5400(200101)69:1<43:ITIMAP>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
MOUSE NRAMP GENE; NATURAL-RESISTANCE; INTRACELLULAR INFECTIONS; PROTEIN; MEMBRANE; ITY/LSH/BCG; EXPRESSION; ACTIVATION; PARASITES; CANDIDATE;
Keywords:
Fe-citrate; PKC; Nramp1(Asp169); cytokines;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Zwilling, BS Ohio State Univ, Dept Microbiol, 484 W 12th Ave, Columbus, OH43210 USA Ohio State Univ 484 W 12th Ave Columbus OH USA 43210 3210 USA
Citazione:
D.E. Kuhn et al., "Iron transport into Mycobacterium avium-containing phagosomes from an Nramp1(Gly169)-transfected RAW264.7 macrophage cell line", J LEUK BIOL, 69(1), 2001, pp. 43-49

Abstract

Nramp1 is an important determinant of innate resistance of macrophages to the growth of intracellular microorganisms. We previously showed that Nramp1 functions to transport iron from the cytoplasm into phagosomes of Mycobacterium avium-infected macrophages. The purpose of this investigation was tofurther characterize the factors that regulate Nramp1-mediated iron transport into phagosomes. Treatment of Nramp1(Gly169) macrophages with the lysomotrophic agents chloroquine or ammonium chloride reduced the import of ironsignificantly, We found that macrophage-activating cytokines, including TNF-alpha, IFN-gamma, IL-1 alpha, aid GM-CSF, when added prior to RI. avium, increased the transport of iron into the phagosome. This increase in iron transport was not a result of all increased amount Of Nramp1 protein in the phagosome nor to new protein synthesis. Treatment of Nramp1(Gly169)-transfected macrophages with inhibitors of protein kinase C (PBC) diminished the import of iron into the phagosomes. Iron import was inhibited by an anti-Nramp1 antibody against the putative fourth outer-loop region of Nramp1 I,nt not by an anti-Nramp1 antibody against the carboxy terminus. The significance of these results on the orientation of Nramp1 ill the phagosome membrane and on the transport of iron is discussed.

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Documento generato il 11/07/20 alle ore 16:19:36