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Titolo:
A MAIZE ZINC-FINGER PROTEIN BINDS THE PROLAMIN BOX IN ZEIN GENE PROMOTERS AND INTERACTS WITH THE BASIC LEUCINE-ZIPPER TRANSCRIPTIONAL ACTIVATOR OPAQUE2
Autore:
VICENTECARBAJOSA J; MOOSE SP; PARSONS RL; SCHMIDT RJ;
Indirizzi:
UNIV CALIF SAN DIEGO,DEPT BIOL,MAIL CODE 0116 LA JOLLA CA 92093 UNIV CALIF SAN DIEGO,DEPT BIOL LA JOLLA CA 92093
Titolo Testata:
Proceedings of the National Academy of Sciences of the United Statesof America
fascicolo: 14, volume: 94, anno: 1997,
pagine: 7685 - 7690
SICI:
0027-8424(1997)94:14<7685:AMZPBT>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
DNA-BINDING; SEQUENCE-ANALYSIS; ENDOSPERM; REGION; DOMAIN; MOTIF; TRANSACTIVATION; IDENTIFICATION; EXPRESSION; ELEMENTS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
32
Recensione:
Indirizzi per estratti:
Citazione:
J. Vicentecarbajosa et al., "A MAIZE ZINC-FINGER PROTEIN BINDS THE PROLAMIN BOX IN ZEIN GENE PROMOTERS AND INTERACTS WITH THE BASIC LEUCINE-ZIPPER TRANSCRIPTIONAL ACTIVATOR OPAQUE2", Proceedings of the National Academy of Sciences of the United Statesof America, 94(14), 1997, pp. 7685-7690

Abstract

The prolamin box (P-box) is a highly conserved 7-bp sequence element (5'-TGTAAAG-3') found in the promoters of many cereal seed storage protein genes. Nuclear factors from maize endosperm specifically interactwith the P-box present in maize prolamin genes (zeins). The presence of the P-box in all zein gene promoters suggests that interactions between endosperm DNA binding proteins and the P-box may play an important role in the coordinate activation of zein gene expression during endosperm development. We have cloned an endosperm-specific maize cDNA, named prolamin-box binding factor (PBF), that encodes a member of the recently described Dof class of plant Cys(2)-Cys(2) zinc-finger DNA binding proteins. When tested in gel shift assays, PBF exhibits the same sequence-specific binding to the P-box as factors present in maize endosperm nuclei. Additionally, PBF interacts in vitro with the basic leucine zipper protein Opaque2, a known transcriptional activator of zeingene expression whose target site lies 20 bp downstream of the P-box in the 22-kDa zein gene promoter. The isolation of the PBF gene provides an essential tool to further investigate the functional role of thehighly conserved P-box in regulating cereal storage protein gene expression.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 00:19:39