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Titolo:
Optimization of background electrolytes for capillary electrophoresis I. Mathematical and computational model
Autore:
Gas, B; Coufal, P; Jaros, M; Muzikar, J; Jelinek, I;
Indirizzi:
Charles Univ, Fac Sci, CZ-12840 Prague 2, Czech Republic Charles Univ Prague Czech Republic 2 , CZ-12840 Prague 2, Czech Republic
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY A
fascicolo: 1-2, volume: 905, anno: 2001,
pagine: 269 - 279
Fonte:
ISI
Lingua:
ENG
Soggetto:
PREDICTING PEAK SYMMETRY; 2 CO-IONS; ZONE-ELECTROPHORESIS; SYSTEM PEAKS; SAMPLE ZONES; CONCENTRATION DISTRIBUTIONS; SHAPE DISTORTIONS; UV DETECTION; SEPARATIONS; ANIONS;
Keywords:
mathematical modelling; optimization; background electrolyte composition;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Gas, B Charles Univ, Fac Sci, Albertov 2030, CZ-12840 Prague 2, Czech Republic Charles Univ Albertov 2030 Prague Czech Republic 2 Czech Republic
Citazione:
B. Gas et al., "Optimization of background electrolytes for capillary electrophoresis I. Mathematical and computational model", J CHROMAT A, 905(1-2), 2001, pp. 269-279

Abstract

A mathematical and computational model is introduced for optimization of background electrolyte systems for capillary zone electrophoresis of anions. The model takes into account mono- or di- or trivalent ions and allows also for modeling of highly acidic or alkaline electrolytes, where a presence of hydrogen and hydroxide ions is significant. At maximum, the electrolyte can contain two co-anions and two counter-cations. The mathematical relations of the model are formulated to enable an easy algorithmization and programming in a computer language. The model assesses the composition of the background electrolyte in the analyte zone, which enables prediction of the parameters of the system that are experimentally available, like the transfer ratio, which is a measure of the sensitivity in the indirect photometric detection or the molar conductivity detection response, which expresses thesensitivity of the conductivity detection. Furthermore, the model also enables the evaluation of a tendency of the analyte to undergo electromigration dispersion and allows the optimization of the composition of the background electrolyte to reach a good sensitivity of detection while still having the dispersion properties in the acceptable range. Although the model presented is aimed towards the separation of anions, it can be straightforwardlyrearranged to serve for simulation of electromigration of cationic analytes. The suitability of the model is checked by inspecting the behavior of a phosphate buffer for analysis of anions. It is shown that parameters of thephosphate buffer when used at neutral and alkaline pH values possess singularities that indicate a possible occurrence of system peaks. Moreover, if the mobility of any analyte of the sample is close to the mobilities of thesystem peaks, the indirect detector signals following the background electrolyte properties will be heavily amplified and distorted. When a specific detector sensitive on presence of the analyte were used, the signal would be almost lost due to the excessive dispersion of the peak. (C) 2001 Published by Elsevier Science B.V.

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Documento generato il 14/07/20 alle ore 03:44:25