Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Optical resolution of asymmetric triacylglycerols by chiral-phase high-performance liquid chromatography
Autore:
Iwasaki, Y; Yasui, M; Ishikawa, T; Irimescu, R; Hata, K; Yamane, T;
Indirizzi:
Nagoya Univ, Grad Sch Bio & Agro Sci, Lab Mol Biotechnol, Chikusa Ku, Nagoya, Aichi 4648601, Japan Nagoya Univ Nagoya Aichi Japan 4648601 a Ku, Nagoya, Aichi 4648601, Japan Nippon Suisan Kaisha Ltd, Cent Res Lab, Hachioji, Tokyo 1920906, Japan Nippon Suisan Kaisha Ltd Hachioji Tokyo Japan 1920906 okyo 1920906, Japan
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY A
fascicolo: 1-2, volume: 905, anno: 2001,
pagine: 111 - 118
Fonte:
ISI
Lingua:
ENG
Soggetto:
RHIZOPUS-DELEMAR LIPASE; FATTY-ACID SPECIFICITY; TRIACYL-SN-GLYCEROLS; ENZYMATIC-SYNTHESIS; STRUCTURED LIPIDS; STEREOSPECIFIC ANALYSIS; CATALYZED SYNTHESIS; PANCREATIC LIPASES; TRIGLYCERIDES; HYDROLYSIS;
Keywords:
triacylglycerol; structured lipids;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Yamane, T Nagoya Univ, Grad Sch Bio & Agro Sci, Lab Mol Biotechnol, Chikusa Ku, FuroCho, Nagoya, Aichi 4648601, Japan Nagoya Univ Furo Cho Nagoya Aichi Japan 4648601 i 4648601, Japan
Citazione:
Y. Iwasaki et al., "Optical resolution of asymmetric triacylglycerols by chiral-phase high-performance liquid chromatography", J CHROMAT A, 905(1-2), 2001, pp. 111-118

Abstract

A simple method for direct optical resolution of some asymmetric triacylglycerols (TGs) has been established. The method employs chiral-phase high-performance liquid chromatography (HPLC). An enantiomeric pair of TGs comprising 1-eicosapentaenoyl-2,3- dicapryroyl-sn-glycerol (ECC) and 1,2-dicapryroyl-3-eicosapentaenoyl-sn-glycerol (CCE) was resolved on a CHIRALCEL OF(TM) or on a CHIRALCEL OD(TM) column. The separation of another pair of asymmetric TGs, 1-docosahexaenoyl-2,3-dicapryroyl-sn-glycerol (DCC) and 1,2-dicapryroyl-3 -docosahexaenoyl-sn-glycerol (CCD), was achieved with the CHIRALCEL OD column. The chiral-phase HPLC method in combination with silver-ion HPLCand high-temperature gas chromatography was used for monitoring two interesterification reactions, whose products were chiral TGs. Interesterification of tricapryloylglycerol with ethyleicosapentaenoate or with ethyldocosahexaenoate was performed using Rhizomucor miehei lipase as the catalyst. The products targeted were the asymmetric pair of TGs, ECC and CCE or DCC and CCD. The amounts of sn-l-substituted products (ECC or DCC) were greater thantheir sn-3-substituted counterparts (CCE or CCD) throughout the reaction period, suggesting that R. miehei lipase had a stereopreference towards the sn-1 position over the sn-3 position. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 23:34:55