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Titolo:
Adrenodoxin reductase homolog (Arh1p) of yeast mitochondria required for iron homeostasis
Autore:
Li, J; Saxena, S; Pain, D; Dancis, A;
Indirizzi:
Univ Penn, Dept Med, Div Hematol Oncol, Philadelphia, PA 19104 USA Univ Penn Philadelphia PA USA 19104 tol Oncol, Philadelphia, PA 19104 USA Univ Penn, Sch Med, Dept Physiol, Philadelphia, PA 19104 USA Univ Penn Philadelphia PA USA 19104 t Physiol, Philadelphia, PA 19104 USA
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 2, volume: 276, anno: 2001,
pagine: 1503 - 1509
SICI:
0021-9258(20010112)276:2<1503:ARH(OY>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
SACCHAROMYCES-CEREVISIAE; SULFUR CLUSTER; MOLECULAR-BIOLOGY; ESSENTIAL GENE; PROTEIN; FRATAXIN; DEFICIENCY; ACONITASE; SYSTEM; CODES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Dancis, A Univ Penn, Dept Med, Div Hematol Oncol, Philadelphia, PA 19104 USA Univ Penn Philadelphia PA USA 19104 Philadelphia, PA 19104 USA
Citazione:
J. Li et al., "Adrenodoxin reductase homolog (Arh1p) of yeast mitochondria required for iron homeostasis", J BIOL CHEM, 276(2), 2001, pp. 1503-1509

Abstract

Arh1p is an essential mitochondrial protein of yeast with reductase activity. Here we show that this protein is involved in iron metabolism. A yeast strain was constructed in which the open reading frame was placed under thecontrol of a galactose-regulated promoter. Protein expression was induced by galactose and repressed to undetectable levels in the absence of galactose, although cells grew quite well in the absence of inducer. Under noninducing conditions, cellular iron uptake was dysregulated, exhibiting a failure to repress in response to medium iron. Iron trafficking within the cell was also disturbed. Exposure of Arh1p-depleted cells to increasing iron concentrations during growth led to drastic increases in mitochondrial iron, indicating a loss of homeostatic control. Activity of aconitase, a prototype Fe-S protein, was deficient at all concentrations of mitochondrial iron, although the protein level was unaltered, Heme protein deficiencies were exacerbated in the iron-loaded mitochondria, suggesting a toxic side effect of accumulated iron. Finally, a time course correlated the cellular depletion of Arh1p with the coordinated appearance of various mutant phenotypes including dysregulated cellular iron uptake, deficiency of Fe-S protein activities in mitochondria and cytoplasm, and deficiency of hemoproteins. Thus, Arh1p is required for control of cellular and mitochondrial iron levels and for the activities of Fe-S cluster proteins.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 18:16:07