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Titolo:
Enhancement of antitumor effect by intratumoral administration of bax genein combination with anticancer drugs in gastric cancer
Autore:
Kim, R; Minami, K; Nishimoto, N; Toge, T;
Indirizzi:
Hiroshima Univ, Res Inst Radiat Biol & Med, Dept Surg Oncol, Minami Ku, Hiroshima 7248553, Japan Hiroshima Univ Hiroshima Japan 7248553 nami Ku, Hiroshima 7248553, Japan
Titolo Testata:
INTERNATIONAL JOURNAL OF ONCOLOGY
fascicolo: 2, volume: 18, anno: 2001,
pagine: 363 - 367
SICI:
1019-6439(200102)18:2<363:EOAEBI>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
CATIONIC LIPID COMPLEXES; CELL LUNG-CANCER; CYTOCHROME-C; PHASE-I; APOPTOSIS; BCL-2; MITOCHONDRIA; CISPLATIN; PLASMID; THERAPY;
Keywords:
bax; apoptosis; intratumoral injection; gastric cancer; anticancer drug;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Kim, R Hiroshima Univ, Res Inst Radiat Biol & Med, Dept Surg Oncol, MinamiKu, 1-2-3 Kasumi, Hiroshima 7248553, Japan Hiroshima Univ 1-2-3 Kasumi Hiroshima Japan 7248553 7248553, Japan
Citazione:
R. Kim et al., "Enhancement of antitumor effect by intratumoral administration of bax genein combination with anticancer drugs in gastric cancer", INT J ONCOL, 18(2), 2001, pp. 363-367

Abstract

Proapototic gene is an important target to enhance the chemotherapeutic effect in cancer cells. Based on in vitro study showing that the introductionof bax gene enhanced the sensitivity to anticancer drugs, we examined whether the intratumoral administration of bax; gene could enhance the antitumor effect in combination with anticancer drugs in gastric cancer. The human gastric cancer cell line, MKN45 was transplanted into nude mice, and the intratumoral administration of bax gene was performed using the bax cDNA plasmid complexed with a cationic lipopolyamine. The enhancement of antitumor effect was examined in the combination with 5-fluorouracil (5-FU) and cisplatin (CDDP). The anticancer drugs were administered intraperitoneally with one third of LD,, four times at 4-day intervals, and the antitumor effect was assessed by the NCI protocol. The expression of bax gene was analyzed by RT-PCR and the apoptotic cell death was assessed by TUNEL method. The intratumoral administration of bax gene alone showed slight anti-tumor effect ascompared to that of control tumor injected with vector alone. The antitumor effect of 5-FU and CDDP was significantly enhanced in the combination with intra-tumoral administration of bax gene as compared to that of CDDP and 5-FU alone (p<0.05, Student's t-test). The enhancement of antitumor effect was associated with the constitutive overexpression of bax gene and with the induction of apoptosis in the tumor treated with anticancer drug and bax gene. These results indicate that the combination therapy of intratumoral administration of bax gene complexed with a cationic lipopolyamine and anticancer drugs may provide us a new strategy for cancer chemotherapy to enhance its therapeutic efficacy in gastric: cancer as termed with gene chemotherapy.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/06/20 alle ore 09:39:22