Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
p53 in SV40-transformed DNA-damaged human cells binds to its cognate sequence but fails to transactivate target genes
Autore:
Technau, A; Wolff, A; Sauder, C; Birkner, N; Brandner, G;
Indirizzi:
Univ Freiburg, Inst Med Mikrobiol & Hyg, Abt Virol, D-79114 Freiburg, Germany Univ Freiburg Freiburg Germany D-79114 Virol, D-79114 Freiburg, Germany
Titolo Testata:
INTERNATIONAL JOURNAL OF ONCOLOGY
fascicolo: 2, volume: 18, anno: 2001,
pagine: 281 - 286
SICI:
1019-6439(200102)18:2<281:PISDHC>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
T-ANTIGEN GENES; WILD-TYPE P53; SIMIAN VIRUS-40; TUMOR-ANTIGEN; MONOCLONAL-ANTIBODIES; SV40; SIMIAN-VIRUS-40; MESOTHELIOMA; FIBROBLASTS; APOPTOSIS;
Keywords:
simian virus 40; SV40; p53; mdm2;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Brandner, G Univ Freiburg, Inst Med Mikrobiol & Hyg, Abt Virol, Hermann Herder Str 11,D-79114 Freiburg, Germany Univ Freiburg Hermann Herder Str 11 Freiburg Germany D-79114
Citazione:
A. Technau et al., "p53 in SV40-transformed DNA-damaged human cells binds to its cognate sequence but fails to transactivate target genes", INT J ONCOL, 18(2), 2001, pp. 281-286

Abstract

Human SV40-transformed cells contain high levels of stabilized p53 of which only a fraction is complexed with the SV40 large tumor antigen (T-antigen). This raises the question whether the p53 which is not complexed with T-antigen retains some biological activity. Two human SV40-transformed cell lines, BEAS and SV80, were investigated. A significant level of constitutive cognate-sequence-specific DNA-binding of p53 was detected by electrophoretic mobility shift assay (EMSA.) of cell extracts. Upon DNA damage by treatment with mitomycin C the DNA-binding activity was increased, as known for cells with wild-type p53. However, in both cell lines, before and after DNA damage, p53 was not able to transactivate a target gene as shown by reportergene assay. Hence, the capability of p53 to bind its cognate sequence is aprerequisite but no proof of p53 transactivating activity. Nuclear p53 levels were not further increased after mitomycin C treatment, occasionally rather slightly decreased, often accompanied by an even larger decrease in amount of T-antigen. In conclusion, SV40-transformation of human cells has caused a loss of essential features of wild-type p53 activity, even in that fraction of p53 not in physical complex with SV40 T-antigen.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 07:51:05