Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Measurement of tumor blood flow using colored dye extraction microspheres in two rat tumor models
Autore:
Iwamaru, A; Watanabe, M; Yu, S; Ohtsuka, T; Horinouchi, H; Kobayashi, K;
Indirizzi:
Keio Univ, Sch Med, Dept Surg, Shinjuku Ku, Tokyo 1608582, Japan Keio Univ Tokyo Japan 1608582 pt Surg, Shinjuku Ku, Tokyo 1608582, Japan
Titolo Testata:
INTERNATIONAL JOURNAL OF ONCOLOGY
fascicolo: 2, volume: 18, anno: 2001,
pagine: 227 - 232
SICI:
1019-6439(200102)18:2<227:MOTBFU>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
ANGIOGENESIS; HYPOXIA; TISSUE;
Keywords:
colored dye extraction microspheres; tumor blood flow; rat tumor model; Sato lung carcinoma; ascites hepatoma LY80;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Iwamaru, A Keio Univ, Sch Med, Dept Surg, Shinjuku Ku, Shinanomachi 35, Tokyo 1608582, Japan Keio Univ Shinanomachi 35 Tokyo Japan 1608582 o 1608582, Japan
Citazione:
A. Iwamaru et al., "Measurement of tumor blood flow using colored dye extraction microspheres in two rat tumor models", INT J ONCOL, 18(2), 2001, pp. 227-232

Abstract

A technique that can measure tumor blood flow easily, accurately and economically is required to study tumor angiogenesis and angiogenesis inhibition. Using dye extraction colored microspheres, we measured tumor blood flow in Sate lung carcinoma (SLC) and ascites hepatoma LY80 in rats. Colored microspheres were infused into tumor-bearing rats via a catheter in the left ventricle. After removal of the tumor and the liver, the tissue samples were dissolved, and the microspheres were isolated. Dye was extracted, and the dye concentration was quantified by spectrophotometry. The dye concentrationper gram of tumor was compared with that per gram of liver as follows (AU = absorbency units): [AU per gram of tumor] / [AV per gram of liver] X 100 = (%). Tumor blood flow corrected for wet weight was calculated as follows:[blood flow,to tumor] = [AU per gram of tumor] X [reference withdrawal rate] / [AU per gram of reference blood]. Tumor blood flow rate was divided bytumor weight to yield ml . min(-1)g(-1). The tumors were also examined histologically, and casts of the tumor vasculature were prepared with siliconerubber. Blood flow 2 weeks after transplantation was equivalent to 1/10 and 1/2 at 1 week in SLC and LY80 tumors, respectively (SLC, P=0.009, n=10; LY80, P=0.05, n=10). These decreases in tumor blood flow were associated with underlying pathological and vascular change. Blood flow in LY80 tumors negatively correlated with tumor volume (P=0.009, n=10). We concluded that the colored microsphere method, initially developed to measure organ blood flow, is also useful for estimating tumor blood flow in rats.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 19:33:12