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Titolo:
In vitro degradation of the Neb-Trypsin Modulating Oostatic Factor (Neb-TMOF) in gut luminal content and hemolymph of the grey fleshfly, Neobellieriabullata
Autore:
Zhu, W; Vandingenen, A; Huybrechts, R; Baggerman, G; De Loof, A; Poulos, CP; Velentza, A; Breuer, M;
Indirizzi:
Katholieke Univ Leuven, Inst Zool, B-3000 Louvain, Belgium Katholieke UnivLeuven Louvain Belgium B-3000 l, B-3000 Louvain, Belgium Univ Patras, Dept Chem, GR-26500 Patras, Greece Univ Patras Patras Greece GR-26500 s, Dept Chem, GR-26500 Patras, Greece
Titolo Testata:
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
fascicolo: 1, volume: 31, anno: 2001,
pagine: 87 - 95
SICI:
0965-1748(200101)31:1<87:IVDOTN>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
ANGIOTENSIN-CONVERTING ENZYME; BIOLOGICAL-ACTIVITY; BIOSYNTHESIS; EXPRESSION; OVARIES; INSECTS; ANALOGS; TESTIS;
Keywords:
Neb-TMOF; Neobelleria bullata; diptera; hemolymph; midgut; proteolytic breakdown; angiotensin converting enzyme (ACE);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
18
Recensione:
Indirizzi per estratti:
Indirizzo: Breuer, M Katholieke Univ Leuven, Inst Zool, B-3000 Louvain, Belgium Katholieke Univ Leuven Louvain Belgium B-3000 Louvain, Belgium
Citazione:
W. Zhu et al., "In vitro degradation of the Neb-Trypsin Modulating Oostatic Factor (Neb-TMOF) in gut luminal content and hemolymph of the grey fleshfly, Neobellieriabullata", INSEC BIO M, 31(1), 2001, pp. 87-95

Abstract

The unblocked hexapeptidic Trypsin Modulating Oostatic Factor of the fleshfly, an inhibitor of both trypsin and ecdysone biosynthesis, resists very well proteolytic breakdown by enzymes present in the lumen of the gut of previtellogenic fleshflies. However, when incubated in hemolymph of adult flies, females and males, its half-life time is a mere 0.5 min. In hemolymph oflast instar larvae, this value increases to about 1.5 min. Whereas PMSF, apotent inhibitor of serine proteases has no effect, captopril and lisinopril, both known to be specific inhibitors of mammalian angiotensin I converting enzyme (ACE), effectively inhibit TMOF breakdown in fly hemolymph. Digestion of Neb-TMOF by recombinant Drosophila AnCE on itself results in identical degradation products as with total hemolymph. In both cases ESI-Qq-oa-Tof mass spectrometry demonstrated the appearance of peptide fragments withthe sequences HPTN, LH and NP. These observations not only confirm the reported presence of circulating ACE-like activity in flies but also strongly suggest that in flies this hemolymph ACE-like activity might be involved inthe regulation of the oostatic activity as exerted by Neb-TMOF. (C) 2001 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 03:02:13