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Titolo:
Effects of neonatal diethylstilbestrol exposure on c-fos and c-jun protooncogene expression in the mouse uterus
Autore:
Yamashita, S; Takayanagi, A; Shimizu, N;
Indirizzi:
Keio Univ, Sch Med, Keio Jr Coll Nursing, Shinjuku Ku, Tokyo 1608582, Japan Keio Univ Tokyo Japan 1608582 Nursing, Shinjuku Ku, Tokyo 1608582, Japan Keio Univ, Sch Med, Dept Mol Biol, Shinjuku Ku, Tokyo 1608582, Japan Keio Univ Tokyo Japan 1608582 ol Biol, Shinjuku Ku, Tokyo 1608582, Japan
Titolo Testata:
HISTOLOGY AND HISTOPATHOLOGY
fascicolo: 1, volume: 16, anno: 2001,
pagine: 131 - 140
SICI:
0213-3911(200101)16:1<131:EONDEO>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
ESTROGEN-RESPONSIVE ELEMENT; MESSENGER-RIBONUCLEIC-ACID; EPIDERMAL GROWTH-FACTOR; IMMEDIATE-EARLY GENES; RAT UTERUS; SIGNALING PATHWAYS; PROTEIN CAUSES; RECEPTOR; MICE; STIMULATION;
Keywords:
c-Fos; c-Jun; diethylstilbestrol; uterus; mouse;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Yamashita, S Keio Univ, Sch Med, Keio Jr Coll Nursing, Shinjuku Ku, 35 Shinanomachi, Tokyo 1608582, Japan Keio Univ 35 Shinanomachi Tokyo Japan 1608582 1608582, Japan
Citazione:
S. Yamashita et al., "Effects of neonatal diethylstilbestrol exposure on c-fos and c-jun protooncogene expression in the mouse uterus", HIST HISTOP, 16(1), 2001, pp. 131-140

Abstract

Quantitative and cell-type-specific expression of c-fos and c-jun genes after 17 beta -estradiol (E2) stimulation, was investigated in the uteri of neonatally diethylstilbestrol (DES)-exposed and ovariectomized adult mice (neoDES-mice), employing Northern blot analysis, immunohistochemistry and in situ hybridization. The c-fos mRNA level before E2 injection (at baseline) was about 2.2-fold higher in neoDES-mice than in vehicle-treated control mice. In controls, E2 treatment transiently increased c-fos mRNA levels, showing a peak value (15.8-fold relative to the baseline) after 2 hours. In neoDES-mice, c-fos mRNA level reached a peak showing a 2.1-fold increase compared with its baseline value 1 hour after E2 injection. Immunohistochemistryand in situ hybridization revealed that c-fos protein (Fos) and mRNA are induced in the epithelium and vascular endothelium in both groups. Most uterine epithelia of neoDES-mice revealed low sensitivity to the c-fos expression after E2 administration compared with those of vehicle-treated controls,whereas few epithelia showed high c-fos mRNA expression even at baseline. The c-jun mRNA concentration in the neoDES-mice uteri at baseline was 70% of that in vehicle-treated controls. At 1 hour after E2 injection, c-jun mRNA levels increased 1.8-fold in controls and 1.3-fold in the neoDES-mice relative to each baseline value. There were no significant differences in the distribution pattern of c-jun protein (Jun) and mRNA in the uteri of eithergroups; E2 stimulated c-jun mRNA expression in the stromal and myometrial cells but suppressed it in the epithelial cells, whereas intensity of c-junimmunostaining increased in the three cell types. The permanent changes inthe expression of estrogen-regulated protooncogenes, c-fos and c-jun genes, by neonatal DES exposure may be responsible for the wide range of abnormalities in the genital tract of mature animals.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/09/20 alle ore 06:16:37