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Titolo:
Long-range comparison of human and mouse SCL loci: Localized regions of sensitivity to restriction endonucleases correspond precisely with peaks of conserved noncoding sequences
Autore:
Gottgens, B; Gilbert, JGR; Barton, LM; Grafham, D; Rogers, J; Bentley, DR; Green, AR;
Indirizzi:
Cambridge Inst Med Res, Wellcome Trust Ctr Mol Mechanisms Dis, Cambridge CB2 2XY, England Cambridge Inst Med Res Cambridge England CB2 2XY bridge CB2 2XY, England Sanger Ctr, Cambridge CB10 1SA, England Sanger Ctr Cambridge England CB10 1SA r Ctr, Cambridge CB10 1SA, England
Titolo Testata:
GENOME RESEARCH
fascicolo: 1, volume: 11, anno: 2001,
pagine: 87 - 97
SICI:
1088-9051(200101)11:1<87:LCOHAM>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
PRIMITIVE MYELOID CELLS; REGULATORY ELEMENTS; TRANSCRIPTIONAL REGULATION; HEMATOPOIETIC LINEAGES; VASCULAR DEVELOPMENT; GENOMIC SEQUENCE; DEVELOPING BRAIN; XENOPUS EMBRYOS; GENE; SCL/TAL-1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: Gottgens, B Cambridge Inst Med Res, Wellcome Trust Ctr Mol Mechanisms Dis,Addenbrookes Hosp Site, Cambridge CB2 2XY, England Cambridge Inst Med Res Addenbrookes Hosp Site Cambridge England CB2 2XY
Citazione:
B. Gottgens et al., "Long-range comparison of human and mouse SCL loci: Localized regions of sensitivity to restriction endonucleases correspond precisely with peaks of conserved noncoding sequences", GENOME RES, 11(1), 2001, pp. 87-97

Abstract

Long-range comparative sequence analysis provides a powerful strategy for identifying conserved regulatory elements. The stem cell leukemia [SCL] gene encodes a bHLH transcription factor with a pivotal role in hemopoiesis and vasculogenesis, and it displays a highly conserved expression pattern. Wepresent here a detailed sequence comparison of 193 kb of the human SCL locus to 234 kb of the mouse SCL locus. Four new genes have been identified together with an ancient mitochondrial insertion in the human locus. The SCL gene is flanked upstream by the SIL gene and downstream by the MAP17 gene in both species, but the gene order is not collinear downstream from MAP17. To facilitate rapid identification of candidate regulatory elements, we have developed a new sequence analysis tool (SynPlot) that automates the graphical display of large-scale sequence alignments. Unlike existing programs, SynPlot can display the locus Features of more than one sequence, thereby indicating the position of homology peaks relative to the structure of all sequences in the alignment. In addition, high-resolution analysis of the chromatin structure of the mouse SCL gene permitted the accurate positioning of localized zones accessible to restriction endonucleases. Zones known to be associated with functional regulatory regions were found to correspond precisely with peaks of human/mouse homology, thus demonstrating that long-range human/mouse sequence comparisons allow accurate prediction of the extent of accessible DNA associated with active regulatory regions.

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Documento generato il 04/04/20 alle ore 02:49:39