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Titolo:
Retinoic acid-mediated gene expression in transgenic reporter zebrafish
Autore:
Perz-Edwards, A; Hardison, NL; Linney, E;
Indirizzi:
Duke Univ, Med Ctr, Dept Cell Biol, Durham, NC 27710 USA Duke Univ DurhamNC USA 27710 d Ctr, Dept Cell Biol, Durham, NC 27710 USA Duke Univ, Med Ctr, Dept Microbiol, Durham, NC 27710 USA Duke Univ DurhamNC USA 27710 d Ctr, Dept Microbiol, Durham, NC 27710 USA Duke Univ, Nicholas Sch Environm, Durham, NC 27710 USA Duke Univ Durham NC USA 27710 Nicholas Sch Environm, Durham, NC 27710 USA
Titolo Testata:
DEVELOPMENTAL BIOLOGY
fascicolo: 1, volume: 229, anno: 2001,
pagine: 89 - 101
SICI:
0012-1606(20010101)229:1<89:RAGEIT>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
VITAMIN-A; EMBRYONIC-DEVELOPMENT; CONE PHOTORECEPTORS; NUCLEAR RECEPTORS; DEFICIENT QUAIL; VENTRAL RETINA; DANIO-RERIO; HINDBRAIN; MICE; DIFFERENTIATION;
Keywords:
retinoic acid; RARE; zebrafish; transgenic; neural tube; retina; heart; diethylaminobenzaldehyde;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
66
Recensione:
Indirizzi per estratti:
Indirizzo: Perz-Edwards, A Duke Univ, Med Ctr, Dept Cell Biol, Durham, NC 27710 USA Duke Univ Durham NC USA 27710 Biol, Durham, NC 27710 USA
Citazione:
A. Perz-Edwards et al., "Retinoic acid-mediated gene expression in transgenic reporter zebrafish", DEVELOP BIO, 229(1), 2001, pp. 89-101

Abstract

Retinoic acid-mediated gene activation is important for normal vertebrate development. The size and nature of retinoic acid make it difficult to identify the precise cellular location of this signaling molecule throughout anembryo. Additionally, retinoic acid (RA) signaling is regulated by a complex combination of receptors, coactivators, and antagonizing proteins. Thus,in order to integrate these signals and identify regions within a whole developing embryo where cells can respond transcriptionally to retinoic acid,we have used a reporter transgenic approach. We have generated several stable lines of transgenic zebrafish which use retinoic acid response elementsto drive fluorescent protein expression. In these zebrafish lines, transgene expression is localized to regions of the neural tube, retina, notochord, somites, heart, pronephric ducts, branchial arches, and jaw muscles in embryos and larvae. Transgene expression can be induced in additional regionsof the neural tube and retina as well as the immature notochord, hatching gland, enveloping cell layer, and fin by exposing embryos to retinoic acid. Treatment with retinoic acid synthase inhibitors, citral and diethylaminobenzaldehyde (DEAB), during neurulation, greatly reduces transgene expression. DEAB treatment of embryos at gastrulation phenocopies the embryonic effects of vitamin A deprivation or targeted disruption of the RA synthase retinaldehyde dehydrogenase-2 in other vertebrates. Together these data suggestthat the reporter expression we see in zebrafish is dependent upon conserved vertebrate pathways of RA synthesis. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/04/20 alle ore 13:09:53