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Titolo:
Activation of human neutrophils by technical toxaphene
Autore:
Gauthier, M; Roberge, CJ; Pelletier, M; Tessier, PA; Girard, D;
Indirizzi:
Univ Quebec, Inst Armand Frappier Sante Humaine, INRS, Pointe Claire, PQ H9R 1G6, Canada Univ Quebec Pointe Claire PQ Canada H9R 1G6 te Claire, PQ H9R 1G6, Canada Assoc Canc Est Quebec, Pointe Claire, PQ H9R 1G6, Canada Assoc Canc Est Quebec Pointe Claire PQ Canada H9R 1G6 PQ H9R 1G6, Canada Univ Laval, CHUQ, Ctr Rech Infectiol, Pointe Claire, PQ H9R 1G6, Canada Univ Laval Pointe Claire PQ Canada H9R 1G6 nte Claire, PQ H9R 1G6, Canada
Titolo Testata:
CLINICAL IMMUNOLOGY
fascicolo: 1, volume: 98, anno: 2001,
pagine: 46 - 53
SICI:
1521-6616(200101)98:1<46:AOHNBT>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-KINASE-C; AQUATIC BIOTA; HUMAN-MILK; APOPTOSIS; EXPRESSION; CONGENERS; PHAGOCYTOSIS; CELLS;
Keywords:
inflammation; neutrophils; organochlorine; phagocytosis; apoptosis; PKCs;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Girard, D Univ Quebec, Inst Armand Frappier Sante Humaine, INRS, 245 Boul Hymus, Pointe Claire, PQ H9R 1G6, Canada Univ Quebec 245 Boul Hymus Pointe Claire PQ Canada H9R 1G6 anada
Citazione:
M. Gauthier et al., "Activation of human neutrophils by technical toxaphene", CLIN IMMUNO, 98(1), 2001, pp. 46-53

Abstract

Toxaphene is a persistent organic pollutant (POP) known to be composed of numerous congeners. Toxaphene technical mixture applied as a pesticide consists of over 800 congeners. Among these, T-2 and T-12 are the two environmentally prevalent forms found in humans. Although toxaphene is known to exert some toxic effects, including potential proinflammatory properties, little is known concerning its action on cells of the human immune system, especially neutrophils. In the present study, we found that toxaphene was not necrotic for human neutrophils incubated for up to 24 h with concentrations ranging from 0.1 to 50 mug/ml. Toxaphene was found to induce neutrophil superoxide production (O-2(-)) in a concentration-dependent manner. The potencyand the kinetics of toxaphene-induced O-2(-) by neutrophils were found to be similar to that of the classical neutrophil agonists phorbol 12-myristate 13-acetate (PIMA). Furthermore, the use of various transduction signal inhibitors (genistein, pertussis toxin, staurosporine, H-7, and HA-1077), suggests that, as for PIMA, toxaphene mediates its effect primarily via PKCs and, to a lesser extend, via tyrosine kinases. In this respect, staurosporine, H-7, and genistein were found to inhibit toxaphene- and PMA-induced O-2(-) production by 52, 72, and 31% and by 63, 62, and 23%, respectively. Toxaphene was also found to significantly enhance neutrophil phagocytosis of opsonized sheep red blood cells and to induce neutrophil apoptosis. The induction of neutrophil apoptosis was paralleled with a decrease in CD16 expression. T-2 and T-12, the two prevalent congeners found in humans, were also found to significantly increase the O-2(-) production in neutrophils at a concentration of 5 mug/ml. We conclude that neutrophils are important targetsfor toxaphene, as this POP can activate O-2(-) production by a PKC- and tyrosine kinase-dependent mechanism, induce phagocytosis, and accelerate the apoptotic rate. This is the first study that focuses on toxaphene/human neutrophil interactions. (C) 2000 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/07/20 alle ore 22:29:21