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Titolo:
Cloning, tissue expression pattern characterization and chromosome localization of human peptide methionine sulfoxide reductase cDNA
Autore:
Hu, PR; Yu, L; Zhang, M; Zheng, LH; Lan, F; Fu, Q; Zhao, SY;
Indirizzi:
Fudan Univ, Sch Life Sci, Inst Genet, State Key Lab Genet Engn, Shanghai 200433, Peoples R China Fudan Univ Shanghai Peoples R China 200433 nghai 200433, Peoples R China
Titolo Testata:
CHINESE SCIENCE BULLETIN
fascicolo: 24, volume: 45, anno: 2000,
pagine: 2251 - 2257
SICI:
1001-6538(200012)45:24<2251:CTEPCA>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
OXIDIZED ALPHA-1-PROTEINASE INHIBITOR; ENZYMATIC REDUCTION; GENE; PROTEINS; PLASMA; FLUID;
Keywords:
peptide methionine sulfoxide reductase; cDNA cloning; expression pattern characterization; human chromosome 8p22-23;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: Yu, L Fudan Univ, Sch Life Sci, Inst Genet, State Key Lab Genet Engn, Shanghai 200433, Peoples R China Fudan Univ Shanghai Peoples R China 200433 200433, Peoples R China
Citazione:
P.R. Hu et al., "Cloning, tissue expression pattern characterization and chromosome localization of human peptide methionine sulfoxide reductase cDNA", CHIN SCI B, 45(24), 2000, pp. 2251-2257

Abstract

Oxidation and reduction of some amino acids are one of the molecular mechanisms for regulating the function of proteins. The oxidation of methionine (Met) to methionine sulfoxide (Met(O)) results in decreasing or loss of thebiological activity of related proteins. It was found that peptide methionine sulfoxide reductase (msrA) can reduce Met(O) to Met and therefore restored the biological function of the oxidized proteins. To reveal the methionine oxidation-reduction mechanism in human body, in this study the cDNA sequence of bovine msrA was used as an information-probe to screen the human EST database. Based on a contig assembled from homologous ESTs, a 1 256-bp human MSRA cDNA was cloned from several human cDNA libraries. The cDNA contains an open reading frame (ORF) of 705 bp in length, which encodes 235 amino acid residues. Homology comparison revealed that human MSRA shares 88% and 61% identities with bovine and Escherichia coli msrA protein respectively. Expression pattern analysis revealed a single 1.6-kb transcript of human MSRA in most human tissues and with highest expression in kidney. By radiation hybrid panel mapping, the gene was localized to human chromosome 8p22-23 between markers D8S518 and D8S550. There are 2 human inherited diseases Keratolytic Winter Erythema and Microcephaly related genes in this region, it is inferred that human MSRA might be the candidate of the two diseases.

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Documento generato il 07/07/20 alle ore 18:48:17