Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Aldosterone and nuclear volume cycling
Autore:
Oberleithner, H; Reinhardt, J; Schillers, H; Pagel, P; Schneider, SW;
Indirizzi:
Univ Munster, Inst Physiol, D-48149 Munster, Germany Univ Munster Munster Germany D-48149 t Physiol, D-48149 Munster, Germany Yale Univ, Sch Med, Dept Cellular & Mol Physiol, New Haven, CT 06510 USA Yale Univ New Haven CT USA 06510 r & Mol Physiol, New Haven, CT 06510 USA
Titolo Testata:
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
fascicolo: 5-6, volume: 10, anno: 2000,
pagine: 429 - 434
SICI:
1015-8987(2000)10:5-6<429:AANVC>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
ATOMIC-FORCE MICROSCOPY; NA+/H+ EXCHANGE; RAPID ACTIVATION; DROSOPHILA-MELANOGASTER; KIDNEY-CELLS; MEMBRANE; PORES;
Keywords:
endothelial cell volume; MDCK-F cell volume; nuclear pore complex; aldosterone receptor; nuclear volume; Xenopus laevis oocyte;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Oberleithner, H Univ Munster, Inst Physiol, Robert Koch Str 27A, D-48149 Munster, Germany Univ Munster Robert Koch Str 27A Munster Germany D-48149
Citazione:
H. Oberleithner et al., "Aldosterone and nuclear volume cycling", CELL PHYS B, 10(5-6), 2000, pp. 429-434

Abstract

The response of target cells to the steroid hormone aldosterone has been divided into acute nongenomic (<10 min) and sustained genomic (>10 min) action. In the light of recent experiments this nomenclature does not hold anymore and should be abandoned. By applying atomic force microscopy (AFM) we observed in living endothelial cells that aldosterone induces cell volume increase in less than 10 minutes. The cell nucleus was identified as the swelling site. Hormone-induced nuclear swelling can reach 15 to 28% of total cell volume dissipating within 30 minutes. This phenomenon could have functional impact on flow resistance in small blood vessels. AFM-investigation of the intracellular signal pathway in nuclear envelope of aldosterone-injected Xenopus laevis oocytes visualizes putative intracellular receptors (40 kDgranules) bound to nuclear pores 2 minutes after hormone injection, with subsequent macromolecule translocation into the nucleus. 15 minutes later macromolecules (800 kD plugs) appear in the central channels of the nuclear pores. The plugs resemble ribonucleoproteins that carry the aldosterone-induced mRNA to the ribosomes. We postulate that steroid-induced nuclear swelling is caused by a shift of receptors/transcription factors from cytoplasm into nucleoplasm followed by gene transcription. Nuclear volume returns to normal when mRNA export through the nuclear pores is finished. Thus, steroid-induced net-movements of macromolecules between intracellular compartmentsinitiate shifts in cell volume compensated by volume regulatory transporters and ion channels in the plasma membrane. Copyright (C) 2000 S. Karger AGBasel.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/20 alle ore 05:52:08