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Titolo:
Expression of cyclooxygenase 2 in human malignant melanoma
Autore:
Denkert, C; Kobel, M; Berger, S; Siegert, A; Leclere, A; Trefzer, U; Hauptmann, S;
Indirizzi:
Charite Hosp, Inst Pathol, D-10117 Berlin, Germany Charite Hosp Berlin Germany D-10117 Inst Pathol, D-10117 Berlin, Germany Charite Hosp, Dept Dermatol & Allergy, D-10117 Berlin, Germany Charite Hosp Berlin Germany D-10117 l & Allergy, D-10117 Berlin, Germany
Titolo Testata:
CANCER RESEARCH
fascicolo: 1, volume: 61, anno: 2001,
pagine: 303 - 308
SICI:
0008-5472(20010101)61:1<303:EOC2IH>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROSTAGLANDIN-G/H SYNTHASE-1; COLON-CANCER CELLS; ASPIRIN USE; CARCINOMA; LUNG; INHIBITION; MACROPHAGES; INVASION; LINES; MICE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Hauptmann, S Charite Hosp, Inst Pathol, D-10117 Berlin, Germany Charite Hosp Berlin Germany D-10117 D-10117 Berlin, Germany
Citazione:
C. Denkert et al., "Expression of cyclooxygenase 2 in human malignant melanoma", CANCER RES, 61(1), 2001, pp. 303-308

Abstract

Cyclooxygenase (COX)-2 is an inducible enzyme involved in production of prostaglandins in inflammatory processes. There is now increasing evidence that a constitutive expression of COX-2 plays a role in development and progression of malignant epithelial tumors. In the present study we investigatedexpression and function of COX-2 in malignant melanoma. Expression of COX-2 was determined by immunohistochemistry in 28 cases of primary skin melanoma and 4 benign nevi. We show that COX-2 was expressed in 26 cases (93%) ofmelanomas, with a moderate to strong expression in 19 cases (68%). Benign nevi as well as normal epithelium sere negative in all cases. A constitutive expression of COX-2 mRNA and protein was found in five melanoma cell lines (A375, MeWo, SK-MeL-13, SK-Mel-28, and IGR-37) by using Northern blot as well as immunoblotting. All melanoma cell, lines produced prostaglandin (PG) E-2 between 468 and 3500 pg/ml as determined by ELISA. Treatment with NS-398 (50 muM), a specific inhibitor of COX-2, suppressed PGE(2) production of all melanoma cell lines by 50-96 %. The IC50 for inhibition of PGE(2) production by NS-398 was determined as 4 muM, indicating that NS-398 acts via inhibition of the COX-2 isoenzyme. We could show that proliferation of melanoma cell lines was not influenced by treatment with NS-398 in concentrations up to 100 muM. However, NS-398 reduced Matrigel invasion of all five malignant melanoma cell lines by 50-68%. Our results indicate that COX-2 is expressed in malignant melanomas and may be involved in regulation of melanoma invasion. It remains to be investigated whether selective inhibitors of COX-2 might be useful for prevention or treatment of malignant melanoma.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/07/20 alle ore 07:31:05