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Titolo:
Differential increase in cerebral cortical glucose oxidative metabolism during rat postnatal development is greater in vivo than in vitro
Autore:
Novotny, EJ; Ariyan, C; Mason, GF; OReilly, J; Haddad, GG; Behar, KL;
Indirizzi:
Yale Univ, Sch Med, Dept Neurol, New Haven, CT 06520 USA Yale Univ New Haven CT USA 06520 ed, Dept Neurol, New Haven, CT 06520 USA Yale Univ, Sch Med, Dept Psychiat, New Haven, CT 06520 USA Yale Univ New Haven CT USA 06520 , Dept Psychiat, New Haven, CT 06520 USA Yale Univ, Sch Med, Dept Pediat, New Haven, CT 06520 USA Yale Univ New Haven CT USA 06520 ed, Dept Pediat, New Haven, CT 06520 USA
Titolo Testata:
BRAIN RESEARCH
fascicolo: 2, volume: 888, anno: 2001,
pagine: 193 - 202
SICI:
0006-8993(20010112)888:2<193:DIICCG>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
REGIONAL ENZYME DEVELOPMENT; ELECTRON-TRANSPORT CHAIN; BLOOD-BRAIN-BARRIER; ENERGY-METABOLISM; AUTORADIOGRAPHIC MEASUREMENT; NMR DETERMINATION; NEURONAL-ACTIVITY; NERVOUS-SYSTEM; IMMATURE RAT; GLUTAMATE;
Keywords:
cerebral development; tricarboxylic acid cycle; oxygen consumption; nuclear magnetic resonance spectroscopy; C-13 isotopes; glutamate; glycolysis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Behar, KL Yale Univ, Sch Med, Dept Neurol, POB 208024, New Haven, CT 06520USA Yale Univ POB 208024 New Haven CT USA 06520 Haven, CT 06520 USA
Citazione:
E.J. Novotny et al., "Differential increase in cerebral cortical glucose oxidative metabolism during rat postnatal development is greater in vivo than in vitro", BRAIN RES, 888(2), 2001, pp. 193-202

Abstract

The steady-state rate of glucose oxidation through the mitochondrial TCA cycle(V-TCA) was measured in acid extracts of 10- and 30-day-old cerebral cortex of rats receiving [1-C-13]glucose intravenously and in neocortical slices superfused in vitro with the same isotope. TCA cycle flux was determined for each age group based on metabolic modeling analysis of the isotopic turnover of cortical glutamate and lactate. The sensitivity of the calculated rates to assumed parameters in the model were also assessed. Between 10 and 30 postnatal days, V-TCA increased by 4.3-fold (from 0.46 to 2.0 mu mol g(-1) min(-1)) in the cortex in vivo, whereas only a 2-fold (from 0.17 to 0.34 mu mol g(-1) min(-1)) increase was observed in neocortical slices. The much greater increase in glucose oxidative metabolism of the cortex measured in vivo over that measured in vitro as the cortex matures suggests that function-related energy demands increase during development, a process that is deficient in the slice as a result of deafferentiation and other mechanisms. (C) 2001 Elsevier Science B.V. All rights reserved.

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Documento generato il 22/01/20 alle ore 09:43:26