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Titolo:
Human CD34(+) stem cells express the hiwi gene, a human homologue of the Drosophila gene piwi
Autore:
Sharma, AK; Nelson, MC; Brandt, JE; Wessman, M; Mahmud, N; Weller, KP; Hoffman, R;
Indirizzi:
Univ Illinois, Coll Med, Dept Med, Hematol Oncol Sect, Chicago, IL 60607 USA Univ Illinois Chicago IL USA 60607 atol Oncol Sect, Chicago, IL 60607 USA Univ Helsinki, Dept Mol Genet, Helsinki, Finland Univ Helsinki Helsinki Finland sinki, Dept Mol Genet, Helsinki, Finland
Titolo Testata:
BLOOD
fascicolo: 2, volume: 97, anno: 2001,
pagine: 426 - 434
SICI:
0006-4971(20010115)97:2<426:HCSCET>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEMATOPOIETIC PROGENITOR CELLS; MURINE MARROW-CELLS; REGULATORY MOLECULES; IN-VIVO; EXPANSION; GERMLINE; FAMILY; PROLIFERATION; HYBRIDIZATION; INTERLEUKIN-3;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
56
Recensione:
Indirizzi per estratti:
Indirizzo: Hoffman, R Univ Illinois, Coll Med, Dept Med, Hematol Oncol Sect, MBRB Room 3150,MC 734,900 S Ashland Ave, Chicago, IL 60607 USA Univ Illinois MBRB Room 3150,MC 734,900 S Ashland Ave Chicago IL USA 60607
Citazione:
A.K. Sharma et al., "Human CD34(+) stem cells express the hiwi gene, a human homologue of the Drosophila gene piwi", BLOOD, 97(2), 2001, pp. 426-434

Abstract

Hematopoietic stem cells (HSCs) are characterized by their dual abilities to undergo differentiation into multiple hematopoietic cell lineages or to undergo self-renewal. The molecular basis of these properties remains poorly understood, Recently the piwi gene was found in the embryonic germline stem cells (GSCs) of Drosophila melanogaster and has been shown to be important in GSC self-renewal, This study demonstrated that hiwi, a novel human homologue of piwi, is also present in human CD34(+) hematopoietic progenitor cells bat not in more differentiated cell populations. Placing CD34(+) cells into culture conditions that supported differentiation and rapid exit from the stem cell compartment resulted in a loss of hiwi expression by day 5 of a 14-day culture period. Expression of the hiwi gene was detected in many developing fetal and adult tissues. By means of 5' RACE cloning methodology, a novel putative full-length hiwi complementary DNA was cloned from human CD34(+) marrow cells. At the amino acid level, the human HIWI protein was 52% homologous to the Drosophila protein. The transient expression of hiwi in the human leukemia cell line KG1 resulted in a dramatic reduction in cellular proliferation. Overexpression of hiwi led to programmed cell death of KG1 cells as demonstrated by the Annexin V assay system. These studies suggest that hiwi maybe an important negative developmental regulator, which, in part, underlies the unique biologic properties associated with hematopoietic stem and progenitor cells. (C) 2001 by The American Society of Hematology.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 15:40:53