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Titolo:
Use of SMART (TM)-generated cDNA for gene expression studies in multiple human tumors
Autore:
Zhumabayeva, B; Diatchenko, L; Chenchik, A; Siebert, PD;
Indirizzi:
Clonetech Labs Inc, Palo Alto, CA 94303 USA Clonetech Labs Inc Palo Alto CA USA 94303 bs Inc, Palo Alto, CA 94303 USA
Titolo Testata:
BIOTECHNIQUES
fascicolo: 1, volume: 30, anno: 2001,
pagine: 158 - 163
SICI:
0736-6205(200101)30:1<158:UOS(CF>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE CHAIN-REACTION; LIBRARY CONSTRUCTION; MESSENGER-RNAS; SEQUENCE TAGS; CLONING; TRANSCRIPTS; AMPLIFICATION; SUPPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Zhumabayeva, B Clonetech Labs Inc, 1020 E Meadow Circle, Palo Alto, CA 94303 USA Clonetech Labs Inc 1020 E Meadow Circle Palo Alto CA USA 94303
Citazione:
B. Zhumabayeva et al., "Use of SMART (TM)-generated cDNA for gene expression studies in multiple human tumors", BIOTECHNIQU, 30(1), 2001, pp. 158-163

Abstract

We demonstrate here that SMART(TM) PCR-amplified cDNAs arrayed on a nylon membrane nle suitable for high-throughput tissue expression profiling when starting biological materials are limited. I We show that SMART cDNA accurately reflects gene expression patterns found in total RNA by comparing the expression level of several target genes in SMART PCR-amplified cDNAs and their corresponding total RNAs. We also arrayed cDNAs from 68 matched tumor and normal samples on a nylon membrane to determine whether SMART PCR-amplified cDNA could be used for detecting differentially expressed genes in these tissues. These arrays containing normalized tumor and normal cDNAs were hybridized with probes for glutathione peroxidase and gelsolin. The hybridization results revealed cancer-related and patient-specific gene expressiondifferences between tumor and normal tissues for these genes. These studies short that SMART PCR-amplified cDNAs maintain the complexity of the original mRNA population and are thus suitable for high-throughput studies to compare the relative abundance of target genes and to detect differentially expressed genes in a wide variety of tissues simultaneously.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 01:44:06