Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Involvement of residues 147VYYEIGK153 in binding of lethal factor to protective antigen of Bacillus anthracis
Autore:
Gupta, P; Singh, A; Chauhan, V; Bhatnagar, R;
Indirizzi:
Jawaharlal Nehru Univ, Ctr Biotechnol, New Delhi 110067, India Jawaharlal Nehru Univ New Delhi India 110067 ol, New Delhi 110067, India
Titolo Testata:
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
fascicolo: 1, volume: 280, anno: 2001,
pagine: 158 - 163
SICI:
0006-291X(20010112)280:1<158:IOR1IB>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
TOXIN CYTOTOXICITY; MAMMALIAN-CELLS; EXPRESSION; MUTAGENESIS; PURIFICATION; J774A.1; PLASMID; KINASE; PLAYS; ACID;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Bhatnagar, R Jawaharlal Nehru Univ, Ctr Biotechnol, New Delhi 110067, India Jawaharlal Nehru Univ New Delhi India 110067 110067, India
Citazione:
P. Gupta et al., "Involvement of residues 147VYYEIGK153 in binding of lethal factor to protective antigen of Bacillus anthracis", BIOC BIOP R, 280(1), 2001, pp. 158-163

Abstract

Anthrax toxin is a complex of protective antigen (PA, 735 aa), lethal factor (LF, 776 aa), and edema factor (EF, 767 aa). PA binds to cell surface receptors and is cleaved by cell surface proteases into PA(63), while LF and EF compete for binding to PA(63). The PA(63)-LF/EF complex is internalized into the cytosol and causes different pathogenic responses in animals and cultured cells. 1-300 amino acid residues of LF have been viewed as the region responsible for the high affinity binding of LF to PA. Amino acid analysis of LF and EF revealed a common stretch of 7 amino acids (147VYYEIGK153). In the present study, each amino acid of this stretch was replaced by alanine at a time. Y148A, Y149A, I151A, and K153A mutants were found to be deficient in their ability to lyse J774A.1 cells and their binding ability to PA(63) was drastically reduced. We propose that these four amino acids play a crucial role in the process of binding of LF to PA(63). (C) 2001 AcademicPress.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 09:34:05