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Titolo:
Androgen receptor isoforms in human and rat prostate
Autore:
Xia, SJ; Hao, GY; Tang, XD;
Indirizzi:
Fudan Univ, Shanghai Peoples Hosp 1, Dept Urol, Shanghai 200080, Peoples RChina Fudan Univ Shanghai Peoples R China 200080 anghai 200080, Peoples RChina Shandong Univ, Affiliated Hosp 2, Dept Urol, Jinan 250033, Peoples R ChinaShandong Univ Jinan Peoples R China 250033 Jinan 250033, Peoples R China
Titolo Testata:
ASIAN JOURNAL OF ANDROLOGY
fascicolo: 4, volume: 2, anno: 2000,
pagine: 307 - 310
SICI:
1008-682X(200012)2:4<307:ARIIHA>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
BREAST-CANCER SAMPLES; LNCAP CELLS; PHOSPHORYLATION; HETEROGENEITY; CLONING;
Keywords:
androgen receptors; isoforms; prostate;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: Xia, SJ Fudan Univ, Shanghai Peoples Hosp 1, Dept Urol, 85 Wu Jin Rd, Shanghai 200080, Peoples R China Fudan Univ 85 Wu Jin Rd Shanghai Peoples R China 200080 s R China
Citazione:
S.J. Xia et al., "Androgen receptor isoforms in human and rat prostate", ASIAN J AND, 2(4), 2000, pp. 307-310

Abstract

Aim: To investigate the androgen receptor (AR) isoforms and its variability of expression in human and rat prostatic tissues. Methods: Human benign prostatic hyperplasia (BPH) and prostatic cancer tissues were obtained from patients undergoing-prostatectomy, and rat ventral prostate was incised 3 days after castration. Forty-one AR-positive BPH specimens, 3 prostatic cancer specimens, and 6 rat prostates were used. After processing at 4 degreesC, the tissues were examined by means of high resolution isoelectric focusing (IEF) technique to determine their AR isoforms. Results: From the prostatic specimens, 3 types of AR isoforms were detected with pI values at 6.5, 6.0, and 5.3. In human BPH tissues, 15/41 (36.6%) specimens showed all the three types of isoforms, while 19/41 (46.3%) showed 2 isoforms at various combinations and 7/41 (17.1%), 1 isoform. For the 3 prostatic cancer specimens, one showed 3 isoforms, one, 2 isoforms, and the other failed to show anyisoform. All rat prostatic tissues showed 2 isoforms at different combinations. Binding of H-3-dihydrotestosterone (DHT) to the isoforms was inhibited by the addition of 100-fold excess of DHT or testosterone, but not progesterone, oestradiol or diethylstilboestrol. Conclusion: AR isoforms are different in different patients. Although their genesis is not clear, the therapeutic implication of the present observation appears to be interesting, that may help clarifying the individual differences in the response to hormonal therapy.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/10/20 alle ore 00:09:34