Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
PrPSc typing by N-terminal sequencing and mass spectrometry
Autore:
Chen, SG; Zou, W; Parchi, P; Gambetti, P;
Indirizzi:
Case Western Reserve Univ, Inst Pathol, Div Neuropathol, Cleveland, OH 44106 USA Case Western Reserve Univ Cleveland OH USA 44106 Cleveland, OH 44106 USA
Titolo Testata:
ARCHIVES OF VIROLOGY
, , anno: 2000, supplemento:, 16
pagine: 209 - 216
SICI:
0304-8608(2000):<209:PTBNSA>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
STRAUSSLER-SCHEINKER-DISEASE; CREUTZFELDT-JAKOB-DISEASE; FATAL FAMILIAL INSOMNIA; HUMAN PRION PROTEIN; DNA POLYMORPHISM; SCRAPIE PRION; CONFORMATIONS; CORRELATE; STRAINS; INDIANA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Chen, SG Case Western Reserve Univ, Inst Pathol, Div Neuropathol, 2085 Adelbert Rd,Cleveland, OH 44106 USA Case Western Reserve Univ 2085 Adelbert RdCleveland OH USA 44106
Citazione:
S.G. Chen et al., "PrPSc typing by N-terminal sequencing and mass spectrometry", ARCH VIROL, 2000, pp. 209-216

Abstract

The heterogeneity of the clinicopathological phenotype in human prion diseases is associated with the presence of the different forms of the abnormalprion protein, PrPSc. We have previously shown that PrPSc in FFI and a subtype of familial CJD linked to the D178N mutation can be distinguished by their difference in gel mobility following proteinase K (PK) treatment. To further characterize the structural difference of PrPSc in familial prion diseases, N-terminal sequencing and mass spectrometry were used to identify the protease cleavage sites in PrPSc extracted from affected brains. We found that the main PK cleavage sites of PrPSc are located at residue 97 in FFI, and residue 82 in both CJD(178) and a GSS subtype linked to the P102L mutation. The differential accessibility to protease in the native PrPSc suggests that PrPSc exist as distinct conformers in different disease states.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/07/20 alle ore 14:54:03