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Titolo:
Fast determination of paraquat residues in water by an optical immunosensor and validation using capillary electrophoresis-ultraviolet detection
Autore:
Mallat, E; Barzen, C; Abuknesha, R; Gauglitz, G; Barcelo, D;
Indirizzi:
CSIC, IIQAB, Dept Environm Chem, ES-08034 Barcelona, Spain CSIC Barcelona Spain ES-08034 t Environm Chem, ES-08034 Barcelona, Spain Univ Tubingen, Inst Phys Chem, D-72076 Tubingen, Germany Univ Tubingen Tubingen Germany D-72076 s Chem, D-72076 Tubingen, Germany Univ London Kings Coll, London W8 7AH, England Univ London Kings Coll London England W8 7AH oll, London W8 7AH, England
Titolo Testata:
ANALYTICA CHIMICA ACTA
fascicolo: 2, volume: 427, anno: 2001,
pagine: 165 - 171
SICI:
0003-2670(20010126)427:2<165:FDOPRI>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
QUATERNARY AMMONIUM HERBICIDES; DIQUAT; DIFENZOQUAT; POTATOES;
Keywords:
optical immunosensor; paraquat residues; capillary zone electrophoresis-ultraviolet detection;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Barcelo, D CSIC, IIQAB, Dept Environm Chem, C Jordi Girona Salgado 18-26, ES-08034 Barcelona, Spain CSIC C Jordi Girona Salgado 18-26 Barcelona Spain ES-08034 ain
Citazione:
E. Mallat et al., "Fast determination of paraquat residues in water by an optical immunosensor and validation using capillary electrophoresis-ultraviolet detection", ANALYT CHIM, 427(2), 2001, pp. 165-171

Abstract

A solid-phase fluoroimmunoassay combined with an optical transducer chemically modified with an analyte derivative coupled to a FIA system was used [A. Brecht, A. Klotz, C. Barzen, G. Gauglitz, R. Harris, G. Quigley, J. Wilkinson, P. Sztajnbok, R. Abuknesha, J. Gascon, A. Oubina, D. Barcelo, Anal. Chim. Acta 362 (1998) 69]. Excitation and collection of fluorescence from fluorescently labelled anti-paraquat antibodies locally bound at the planar interface allows the measurement of the fluorescent signal which is indirectly related to the paraquat concentration of the sample. Matrix effects on the immunosensor response were observed, thus leading to the following detection limits, 0.01 and 0.06 mug l(-1), when analyzing paraquat in MilliQ and in river water, respectively, The validation of the biosensor was carriedout analyzing paraquat samples by capillary zone electrophoresis with ultraviolet detection (CZE-UV) at 214 nm. Preconcentration of the samples priorto their injection in the capillary electrophoresis were performed using the automated solid-phase extraction system (ASPEC-XL). Paraquat samples were adjusted at pH 9 and were percolated through a silica cartridge, subsequent elution was carried out using a mixture of hydrochloric acid and methanol and afterwards samples were evaporated and injected. This optical immunosensor, also called River ANAlyzer (RIANA) with an optical immunosensor can be applied for fast monitoring of paraquat in river water samples being the total analysis time of 15 min. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 14:34:51