Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Effects of endotoxin on neutrophil-mediated I/R injury in isolated perfused rat hearts
Autore:
Lipton, BP; Bautista, AP; Delcarpio, JB; McDonough, KH;
Indirizzi:
Louisiana State Univ, Med Ctr, Dept Physiol & Cell Biol, New Orleans, LA 70112 USA Louisiana State Univ New Orleans LA USA 70112 , New Orleans, LA 70112 USA Louisiana State Univ, Med Ctr, Dept Anat, New Orleans, LA 70112 USA Louisiana State Univ New Orleans LA USA 70112 , New Orleans, LA 70112 USA
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
fascicolo: 2, volume: 280, anno: 2001,
pagine: H802 - H811
SICI:
0363-6135(200102)280:2<H802:EOEONI>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
MYOCARDIAL REPERFUSION INJURY; ENDOTHELIAL CELL RECOGNITION; SOLUBLE E-SELECTIN; POLYMORPHONUCLEAR LEUKOCYTES; BACTERIAL LIPOPOLYSACCHARIDE; INDUCED TOLERANCE; ISCHEMIA; MAC-1; EXPRESSION; ADHESION;
Keywords:
left ventricular function; neutrophils; endotoxin; ischemia-reperfusion;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: McDonough, KH Louisiana State Univ, Med Ctr, Dept Physiol, 1901 Perdido St, New Orleans,LA 70112 USA Louisiana State Univ 1901 Perdido St New OrleansLA USA 70112
Citazione:
B.P. Lipton et al., "Effects of endotoxin on neutrophil-mediated I/R injury in isolated perfused rat hearts", AM J P-HEAR, 280(2), 2001, pp. H802-H811

Abstract

With the use of a syngeneic model, we demonstrate that rat polymorphonuclear neutrophils (PMNs) exacerbate ischemia-reperfusion injury in the isolated rat heart. However, PMNs (19 x 10(6) cells) from lipopolysaccharide (LPS)treated rats (LPS-PMNs; 100 mg/kg administered 7 h before exsanguination) induce less reperfusion injury in the isolated heart. Average recovery of left ventricular developed pressure after 20 min of ischemia and 60 min of reperfusion was 51 +/- 4% in hearts receiving PMNs from saline-treated control rats (saline-PMNs) versus 78 +/- 2% in hearts receiving LPS-PMNs. Ischemic hearts reperfused with LPS-PMNs recovered to the same extent as did hearts reperfused with Krebs buffer only. LPS-PMNs and saline-PMNs showed no difference in basal or phorbol ester-induced superoxide production. Whereas twice the number of LPS-PMNs was positive for nitroblue tetrazolium, the percent positive for L-selectin, a receptor integral in PMN-adhesion to endothelium, was 50% less in LPS-PMNs than in controls. After reperfusion, three-fourths of the saline-PMNs remained within the hearts, whereas only one-fourth of LPS-PMNs were trapped. These data suggest that PMNs from LPS-treatedrats do not exacerbate ischemia-reperfusion injury as do control PMNs, possibly, due to impaired PMN adhesion to endothelium as a result of decreasedL-selectin receptors.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 13/07/20 alle ore 07:25:49