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Titolo:
Inhibition of p38 MAPK alpha/beta reduces ischemic injury and does not block protective effects of preconditioning
Autore:
Schneider, S; Chen, WN; Hou, J; Steenbergen, C; Murphy, E;
Indirizzi:
NIEHS, Lab Signal Transduct, NIH, Res Triangle Pk, NC 27709 USA NIEHS ResTriangle Pk NC USA 27709 ct, NIH, Res Triangle Pk, NC 27709 USA Univ N Carolina, Chapel Hill, NC 27516 USA Univ N Carolina Chapel Hill NCUSA 27516 olina, Chapel Hill, NC 27516 USA Duke Univ, Med Ctr, Durham, NC 27710 USA Duke Univ Durham NC USA 27710Duke Univ, Med Ctr, Durham, NC 27710 USA
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
fascicolo: 2, volume: 280, anno: 2001,
pagine: H499 - H508
SICI:
0363-6135(200102)280:2<H499:IOPMAR>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACTIVATED PROTEIN-KINASES; PERFUSED RAT HEARTS; N-TERMINAL KINASES; CELL INJURY; STRESS; PHOSPHORYLATION; REPERFUSION; MYOCARDIUM; INTERLEUKIN-1; ACCUMULATION;
Keywords:
SB-202190; P-31 nuclear magnetic resonance; necrosis; intracellular pH; left ventricular developed pressure;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Murphy, E NIEHS, Lab Signal Transduct, NIH, Mail Drop D2-03,POB 12233, ResTriangle Pk, NC 27709 USA NIEHS Mail Drop D2-03,POB 12233 Res Triangle Pk NC USA 27709 USA
Citazione:
S. Schneider et al., "Inhibition of p38 MAPK alpha/beta reduces ischemic injury and does not block protective effects of preconditioning", AM J P-HEAR, 280(2), 2001, pp. H499-H508

Abstract

We examined the effect of inhibition of p38 mitogen-activated protein kinase (MAPK) alpha/beta during ischemia and preconditioning by using the inhibitor SB-202190. Isolated rat hearts were perfused with Krebs-Henseleit buffer, while left ventricular developed pressure (LVDP) and P-31 nuclear magnetic resonance spectra were acquired continuously. After 20 min of ischemia and 25 min of reperfusion, recovery of LVDP in untreated hearts was 32 +/- 4%, whereas hearts treated with SB-202190 5 min before ischemia recovered 59 +/- 7% of their pretreatment LVDP. Preconditioning improved functional recovery to 65 +/- 5%, which was unaffected by SB-202190 treatment, added either throughout the preconditioning protocol (56 +/- 5% recovery) or during the final reperfusion period of preconditioning (71 +/- 11% recovery). Necrosis was assessed after 40 min of ischemia and 2 h of reperfusion using 2,3,5-triphenyltetrazolium chloride (TTC) staining and creatine kinase release. The untreated group had 54 +/- 8% necrotic myocardium, whereas the SB-202190-treated group had 32 +/- 7% and the preconditioned group had 21 +/- 4% necrotic tissue by TTC staining.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 12:04:41