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Titolo:
Expression of extracellular calcium-sensing receptor in human osteoblasticMG-63 cell line
Autore:
Yamaguchi, T; Chattopadhyay, N; Kifor, O; Ye, CP; Vassilev, PM; Sanders, JL; Brown, EM;
Indirizzi:
Harvard Univ, Brigham & Womens Hosp, Sch Med, Dept Med,Endocrine HypertensDiv, Boston, MA 02115 USA Harvard Univ Boston MA USA 02115 crine HypertensDiv, Boston, MA 02115 USA
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
fascicolo: 2, volume: 280, anno: 2001,
pagine: C382 - C393
SICI:
0363-6143(200102)280:2<C382:EOECRI>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
OUTWARD K+ CHANNEL; BOVINE PARATHYROID CELLS; CA2+-SENSING RECEPTOR; (CA-O(2+))-SENSING RECEPTOR; FUNCTIONAL EXPRESSION; OSTEOSARCOMA CELLS; BONE-FORMATION; SECRETION; CLONING; NEURONS;
Keywords:
G protein-coupled receptor; potassium channel; Northern analysis; reverse transcriptase-polymerase chain reaction; Western analysis; immunocytochemistry;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Brown, EM Harvard Univ, Brigham & Womens Hosp, Sch Med, Dept Med,EndocrineHypertensDiv, 221 Longwood Ave, Boston, MA 02115 USA Harvard Univ 221 Longwood Ave Boston MA USA 02115 , MA 02115 USA
Citazione:
T. Yamaguchi et al., "Expression of extracellular calcium-sensing receptor in human osteoblasticMG-63 cell line", AM J P-CELL, 280(2), 2001, pp. C382-C393

Abstract

We have previously shown the expression of the extracellular calcium (Ca-o(2+))-sensing receptor (CaR) in osteoblast-like cell lines, and others havedocumented its expression in sections of murine, bovine, and rat bone. Theexistence of the CaR in osteoblasts remains controversial, however, since some studies have failed to document its expression in the same osteoblast-like cell lines. The goals of the present study were twofold. 1) We sought to determine whether the CaR is expressed in the human osteoblast-like cellline, MG-63, which has recently been reported by others not to express this receptor. 2) We investigated whether the CaR, if present in MG-63 cells, is functionally active, since most previous studies have not proven the role of the CaR in mediating known actions of Ca-o(2+) on osteoblast-like cells. We used immunocytochemistry and Western blotting with the specific, affinity-purified anti-CaR antiserum 4637 as well as Northern blot analysis andRT-PCR using a riboprobe and PCR primers specific for the human CaR, respectively, to show readily detectable CaR protein and mRNA expression in MG-63 cells. Finally, we employed the patch-clamp technique to show that an elevation in Ca-o(2+) as well as the specific, allosteric CaR activator NPS R-467 (0.5 muM), but not its less active stereoisomer NPS S-467 (0.5 muM), activate an outward K+ channel in MG-63 cells, strongly suggesting that the CaR in MG-63 cells is not only expressed but is functionally active.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/07/20 alle ore 01:12:56