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Titolo:
Regulatory pathways and uptake of L-DOPA by capillary cerebral endothelialcells, astrocytes, and neuronal cells
Autore:
Sampaio-Maia, B; Serrao, MP; Soares-da-Silva, P;
Indirizzi:
Fac Med, Inst Pharmacol & Therapeut, P-4200319 Porto, Portugal Fac Med Porto Portugal P-4200319 & Therapeut, P-4200319 Porto, Portugal
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
fascicolo: 2, volume: 280, anno: 2001,
pagine: C333 - C342
SICI:
0363-6143(200102)280:2<C333:RPAUOL>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
CAPACITATIVE CA2+ ENTRY; AMINO-ACID-TRANSPORT; BLOOD-BRAIN-BARRIER; CA2+-INHIBITABLE ADENYLYL-CYCLASE; C6-2B GLIOMA-CELLS; NEUROBLASTOMA-CELLS; STRIATAL NEURONS; P-GLYCOPROTEIN; RAT; NEUROTOXICITY;
Keywords:
L-3,4-dihydroxyphenylalanine;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Soares-da-Silva, P Fac Med, Inst Pharmacol & Therapeut, P-4200319 Porto, Portugal Fac Med Porto Portugal P-4200319 0319 Porto, Portugal
Citazione:
B. Sampaio-Maia et al., "Regulatory pathways and uptake of L-DOPA by capillary cerebral endothelialcells, astrocytes, and neuronal cells", AM J P-CELL, 280(2), 2001, pp. C333-C342

Abstract

We examined the nature and regulation of the inward L-3,4-dihydroxyphenylalanine (L-DOPA) transporter in rat capillary cerebral endothelial (RBE4) cells, type 1 astrocytes (DI TNC1), and Neuro-2a neuroblastoma cells. In all three cell types, the inward transfer of L-DOPA was largely promoted through the 2-aminobicyclo-(2,2,1)-heptane-2-carboxylic acid-sensitive and sodium-independent L-type amino acid transporter. Only in DI TNC1 cells was the effect of maneuvers that increase intracellular cAMP levels accompanied by increases in L-DOPA uptake. Also, only in DI TNC1 cells was the effect of the guanylyl cyclase inhibitor LY-83583 accompanied by a 65% increase in L-DOPA accumulation, whereas the nitric oxide donor sodium nitroprusside produced a 25% decrease in L-DOPA accumulation. In all three cell types, the Ca2+/calmodulin inhibitors calmidazolium and trifluoperazine inhibited L-DOPA uptake in a noncompetitive manner. Thapsigargin (1 and 3 muM) and A-23187 (1and 3 muM) failed to alter L-DOPA accumulation in RBE4 and Neuro-2a cells but markedly increased L-DOPA uptake in DI TNC1 cells. We concluded that L-DOPA in RBE4, DI TNC1, and Neuro-2a cells is transported through the L-typeamino acid transporter and appears to be under the control of Ca2+/calmodulin- mediated pathways. Astrocytes, however, are endowed with other processes that appear to regulate the accumulation of L-DOPA, responding positively to increases in intracellular Ca2+ and cAMP and to decreases in cGMP.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 02:40:33