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Titolo:
Herpesvirus saimiri pathogenicity enhanced by thymidine kinase of herpes simplex virus
Autore:
Hiller, C; Tamguney, G; Stolte, N; Matz-Rensing, K; Lorenzen, D; Hor, S; Thurau, M; Wittmann, S; Slavin, S; Fickenscher, H;
Indirizzi:
Univ Erlangen Nurnberg, Inst Klin & Mol Virol, D-91054 Erlangen, Germany Univ Erlangen Nurnberg Erlangen Germany D-91054 -91054 Erlangen, Germany German Primate Ctr, Div Virol & Immunol, D-37077 Gottingen, Germany GermanPrimate Ctr Gottingen Germany D-37077 D-37077 Gottingen, Germany Div Primate Vet Med & Primate Husb, D-37077 Gottingen, Germany Div PrimateVet Med & Primate Husb Gottingen Germany D-37077 en, Germany Hadassah Univ Hosp, Dept Bone Marrow Transplantat, IL-91220 Jerusalem, Israel Hadassah Univ Hosp Jerusalem Israel IL-91220 IL-91220 Jerusalem, Israel
Titolo Testata:
VIROLOGY
fascicolo: 2, volume: 278, anno: 2000,
pagine: 445 - 455
SICI:
0042-6822(200012)278:2<445:HSPEBT>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
BONE-MARROW TRANSPLANTATION; GRAFT-VERSUS-LEUKEMIA; T-CELL CLONES; PERIPHERAL-BLOOD LYMPHOCYTES; SUBGROUP-C STRAINS; GAMMA-DELTA; TRANSGENIC MICE; GROWTH TRANSFORMATION; FUNCTIONAL PHENOTYPE; GENE-TRANSFER;
Keywords:
aciclovir; Callithrix jacchus; common marmosets; ganciclovir; herpes simplex virus; herpesvirus saimiri; pathogenicity; suicide gene; T-cell leukemia; thymidine kinase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
62
Recensione:
Indirizzi per estratti:
Indirizzo: Fickenscher, H Univ Erlangen Nurnberg, Inst Klin & Mol Virol, Schlossgarten 4, D-91054 Erlangen, Germany Univ Erlangen Nurnberg Schlossgarten 4 Erlangen Germany D-91054
Citazione:
C. Hiller et al., "Herpesvirus saimiri pathogenicity enhanced by thymidine kinase of herpes simplex virus", VIROLOGY, 278(2), 2000, pp. 445-455

Abstract

Herpesvirus saimiri can be used as an efficient gene expression Vector forhuman T lymphocytes and thus may allow applications in experimental leukemia therapy. We constructed recombinant viruses for the functional expression of the thymidine kinase (TK) of herpes simplex virus type 1 (HSV) as a suicide gene. These viruses reliably allowed the targeted elimination of transduced nonpermissive human T cells in vitro after the administration of ganciclovir. To test the reliability of this function under the most stringentpermissive conditions, in this study we analyzed the influence of the prodrugs ganciclovir and acyclovir in common marmosets on the acute leukemogenesis induced by either wild-type herpesvirus saimiri C488 or by a recombinant derivative expressing TK of HSV. Antiviral drug treatment did not influence the rapid development of acute disease. In contrast, the presence of theHSV tk gene resulted in a faster disease progression. In addition, HSV TK-expressing viruses showed faster replication than wild-type virus in culture at low serum concentrations. Thus, HSV TK accelerates the replication of herpesvirus saimiri and enhances its pathogenicity. This should be generally considered when HSV TK is applied as a transgene in replication-competentDNA virus vectors for gene therapy. (C) 2000 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/06/20 alle ore 02:27:20