Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Primitive human HPCs are better maintained and expanded in vitro at 1 percent oxygen than at 20 percent
Autore:
Ivanovic, Z; Dello Sbarba, P; Trimoreau, F; Faucher, JL; Praloran, V;
Indirizzi:
Univ Limoges, Fac Med, Lab Expt Hematol, Limoges, France Univ Limoges Limoges France Fac Med, Lab Expt Hematol, Limoges, France Univ Florence, Dept Expt Pathol & Oncol, Florence, Italy Univ Florence Florence Italy Dept Expt Pathol & Oncol, Florence, Italy
Titolo Testata:
TRANSFUSION
fascicolo: 12, volume: 40, anno: 2000,
pagine: 1482 - 1488
SICI:
0041-1132(200012)40:12<1482:PHHABM>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEMATOPOIETIC STEM-CELLS; GROWTH-FACTOR REQUIREMENT; EX-VIVO EXPANSION; PROGENITOR CELLS; CD34(+) CELLS; CORD-BLOOD; MODERATE HYPOXIA; IN-VITRO; PROLIFERATION; CULTURE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Ivanovic, Z Univ Bordeaux 2, Lab Univ Hematol, 146 Rue Leo Saignat, F-33076 Bordeaux, France Univ Bordeaux 2 146 Rue Leo Saignat Bordeaux France F-33076 e
Citazione:
Z. Ivanovic et al., "Primitive human HPCs are better maintained and expanded in vitro at 1 percent oxygen than at 20 percent", TRANSFUSION, 40(12), 2000, pp. 1482-1488

Abstract

BACKGROUND: The liquid culture of murine bone marrow cells at 1-percent oxygen maintains the balance between primative progenitor cell renewal and clonogenic progenitor expansion better than that at 20-percent oxygen. These results are of potential interest for the ex vivo expansion of human progenitor cells, as low O-2 tension could preserve the engraftment potential of cultured apheresis products. STUDY DESIGN AND METHODS: G-CSF-mobilized blood cells collected by apheresis, now the main source of progenitor cells for autologous transplantation,were cultured at 1-percent and 20-percent O-2 for 7 days in serum-free liquid cultures in the presence of IL-3 and SCF (5 ng/mL). The growth of the clonogenic progenitors (CFU-GM, BFU-E, CFU-Mix) and of the more primitive human HPCs that are capable of generating clongenic progenitors in secondary liquid culture, as well as the proliferation and differentiation of total and CD34+ cells, was analyzed. RESULTS: The expansion of CD34+ cells and of clonogenic progenitors was significantly lower in liquid cultures at 1-percent O-2 than at 20-percent O-2. On the contrary, the primitive human HPCs were better maintained and expanded at 1-percent O-2, although the number of CD34+ cells remaining quiescent was lower. After 7 days of liquid culture at 1-percent or 20-percent O-2 the percentage of CD34+ cells was similar. However, the CD34+ cells that divided more than four times (PKH2 staining) were more numerous in liquid cultures incubated at 1-percent O-2. CONCLUSION: When cultured at 1-percent O-2 for 7 days in presence of IL-3 and SCF, the CD34+ cells present in apheresis components underwent more cell divisions and better maintained their primitive progenitor cell potential. As suggested by previous results in mice, our data on human cells emphasize the potential interest of cultures at low O-2 tension (1%) for cell therapy protocols aimed at expanding primitive HPCs in autografts.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/06/20 alle ore 08:13:16