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Titolo:
Three distinct types of GnRH receptor characterized in the bullfrog
Autore:
Wang, L; Bogerd, J; Choi, HS; Seong, JY; Soh, JM; Chun, SY; Blomenrohr, M; Troskie, BE; Millar, RP; Yu, WH; McCann, SM; Kwon, HB;
Indirizzi:
Chonnam Natl Univ, Hormone Res Ctr, Kwangju 500757, South Korea Chonnam Natl Univ Kwangju South Korea 500757 Kwangju 500757, South Korea Chonnam Natl Univ, Dept Biol, Kwangju 500757, South Korea Chonnam Natl Univ Kwangju South Korea 500757 Kwangju 500757, South Korea Univ Utrecht, Dept Expt Zool, Res Grp Comparat Endocrinol, NL-3584 Utrecht, Netherlands Univ Utrecht Utrecht Netherlands NL-3584 l, NL-3584 Utrecht, Netherlands Univ Cape Town, Dept Chem Pathol, Res Unit Mol Reprod Endocrinol, ZA-7925 Cape Town, South Africa Univ Cape Town Cape Town South Africa ZA-7925 25 Cape Town, South Africa MRC, Reprod Biol Unit, Edinburgh EH3 9ET, Midlothian, Scotland MRC Edinburgh Midlothian Scotland EH3 9ET h EH3 9ET, Midlothian, Scotland Louisiana State Univ, Pennington Biomed Res Ctr, Baton Rouge, LA 70808 USALouisiana State Univ Baton Rouge LA USA 70808 , Baton Rouge, LA 70808 USA
Titolo Testata:
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
fascicolo: 1, volume: 98, anno: 2001,
pagine: 361 - 366
SICI:
0027-8424(20010102)98:1<361:TDTOGR>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
GONADOTROPIN-RELEASING-HORMONE; LIGAND-BINDING; TERMINAL TAIL; EXPRESSION; PITUITARY; SEQUENCE; CLONING; FORMS; PHOSPHORYLATION; INTERNALIZATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Kwon, HB Chonnam Natl Univ, Hormone Res Ctr, Kwangju 500757, South Korea Chonnam Natl Univ Kwangju South Korea 500757 00757, South Korea
Citazione:
L. Wang et al., "Three distinct types of GnRH receptor characterized in the bullfrog", P NAS US, 98(1), 2001, pp. 361-366

Abstract

It has been proposed recently that two types of GnRH receptors (GnRHR) exist in a particular species. Here we present data demonstrating that at least three types of GnRHR are expressed in a single diploid species, the bullfrog. Three different cDNAs. encoding distinct types of bullfrog GnRHR (bfGnRHR-1, bfGnRHR-2, and bfGnRHR-3), were isolated from pituitary and hindbrain of the bullfrog. BfGnRHR-1 mRNA was expressed predominantly in pituitary,whereas bfGnRHR-2 and -3 mRNAs were expressed in brain. The bfGnRHR-1, bfGnRHR-2, and bfGnRHR-3 proteins have an amino acid identity of approximate to 30% to approximate to 35% with mammalian GnRHRs and approximate to 40% toapproximate to 50% with nonmammalian GnRHRs. Interestingly, bfGnRHR-2 has an 85% amino acid homology with Xenopus GnRHR. Less than 53% amino acid identity was observed among the three bfGnRHRs. All isolated cDNAs encode functional receptors because their transient expression in COS-7 cells resultedin a ligand-dependent increase in inositol phosphate production. Notably, all three receptors exhibited a differential ligand selectivity. For all receptors, cGnRH-II has a higher potency than mGnRH. In addition, salmon GnRHalso has a strikingly high potency to stimulate all three receptors. In conclusion, we demonstrated the presence of three GnRHRs in the bullfrog. Their expression in pituitary and brain suggests that bfGnRHRs play an important role in the regulation of reproductive functions in the bullfrog.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 08:10:51