Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Bid acts on the permeability transition pore complex to induce apoptosis
Autore:
Zamzami, N; El Hamel, C; Maisse, C; Brenner, C; Munoz-Pinedo, C; Belzacq, AS; Costantini, P; Vieira, H; Loeffler, M; Molle, G; Kroemer, G;
Indirizzi:
Inst Gustave Roussy, CNRS, UMR 1599, F-94805 Villejuif, France Inst Gustave Roussy Villejuif France F-94805 , F-94805 Villejuif, France Univ Technol Compiegne, UMR 6022, CNRS, F-60205 Compiegne, France Univ Technol Compiegne Compiegne France F-60205 -60205 Compiegne, France Fac Sci, IFRMP23, UMR 6522, CNRS, F-76821 Mt St Aignan, France Fac Sci MtSt Aignan France F-76821 , CNRS, F-76821 Mt St Aignan, France CSIC, Inst Parasitol & Biomed, Granada 18001, Spain CSIC Granada Spain 18001 , Inst Parasitol & Biomed, Granada 18001, Spain
Titolo Testata:
ONCOGENE
fascicolo: 54, volume: 19, anno: 2000,
pagine: 6342 - 6350
SICI:
0950-9232(200012)19:54<6342:BAOTPT>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYTOCHROME-C RELEASE; BCL-2 FAMILY; ISOLATED-MITOCHONDRIA; BAX; CHANNEL; PROTEINS; MEMBRANES; YEAST; VDAC;
Keywords:
apoptosis; Bcl-2; mitochondria; permeability transition; t-Bid;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Kroemer, G Inst Gustave Roussy, CNRS, UMR 1599, 39 Rue Camille Desmoulins,F-94805 Villejuif, France Inst Gustave Roussy 39 Rue Camille Desmoulins Villejuif France F-94805
Citazione:
N. Zamzami et al., "Bid acts on the permeability transition pore complex to induce apoptosis", ONCOGENE, 19(54), 2000, pp. 6342-6350

Abstract

Similar to most if not all pro-apoptotic members of the Bcl-2 family, Bid land its truncated product t-Bid) triggers cell death via mitochondrial membrane permeabilization (MMP). This effect can be monitored in intact cells,upon microinjection of recombinant Bid protein into the cytoplasm, as wellas in purified mitochondria, upon addition of Bid protein. Here we show that Bid-induced MMP can be inhibited, both in cells and in the cell-free system, by three pharmacological inhibitors of the permeability transiton porecomplex (PTPC), namely cyclosporin A, N-methyl-4-Val-cyclosporin A, and bongkrekic acid (a ligand of the adenine nucleotide translocase, ANT, one of the PTPC components). Bid effects on synthetic membranes were studied either in proteoliposomes or in synthetic bilayers subjected to electrophysiological measurements. Full length Bid preferentially permeabilizes membranes and induces the formation of large conductance channels at neutral pH, when added to liposomes or bilayers containing both purified ANT and Bax, yet has no or little effect combined with ANT or Bax alone. t-Bid acts on membranes containing ANT alone with the same efficiency as on those containing both ANT and Bax. These results suggest that the proapoptotic effects of Bid are mediated, at least in part, by its functional interaction with ANT, one of the major components of PTPC.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/07/20 alle ore 19:04:38