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Titolo:
Anti-HIV-1 activity by a triple-helix forming oligonucleotides targeted topolypurine tract on viral RNA
Autore:
Hiratou, T; Tsukahara, S; Miyano-Kurosaki, W; Takai, K; Saito, T; Yamamoto, N; Takaku, H;
Indirizzi:
Dept Ind Chem, Chiba 2750016, Japan Dept Ind Chem Chiba Japan 2750016Dept Ind Chem, Chiba 2750016, Japan Chiba Inst Technol, Chiba 2750016, Japan Chiba Inst Technol Chiba Japan 2750016 nst Technol, Chiba 2750016, Japan Yamanouchi Pharmaceut Co Ltd, Inst Consumer Healthcare, Itabashi Ku, Tokyo1748612, Japan Yamanouchi Pharmaceut Co Ltd Tokyo Japan 1748612 Ku, Tokyo1748612, Japan Tokyo Med & Dent Univ, Sch Med, Dept Microbiol, Bunkyo Ku, Tokyo 1138519, Japan Tokyo Med & Dent Univ Tokyo Japan 1138519 unkyo Ku, Tokyo 1138519, Japan
Titolo Testata:
NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS
fascicolo: 10-12, volume: 19, anno: 2000,
pagine: 1721 - 1734
SICI:
1525-7770(2000)19:10-12<1721:AABATF>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-IMMUNODEFICIENCY-VIRUS; DEPENDENT DNA POLYMERASE; PHOSPHOROTHIOATE OLIGODEOXYNUCLEOTIDE; REVERSE TRANSCRIPTION; HIV-1 GP120; BINDING; INHIBITION; VIRIONS; GUANINE; ANALOGS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Takaku, H Dept Ind Chem, Chiba 2750016, Japan Dept Ind Chem Chiba Japan 2750016 d Chem, Chiba 2750016, Japan
Citazione:
T. Hiratou et al., "Anti-HIV-1 activity by a triple-helix forming oligonucleotides targeted topolypurine tract on viral RNA", NUCLEOS NUC, 19(10-12), 2000, pp. 1721-1734

Abstract

Reverse transcription of HIV-1 into double-stranded DNA involves initiation of plus-strand DNA synthesis at the polypurine tract, PPT, by reverse transcriptase (RT). The PPT is a possible target for triple-helix formation. We show the effects of triple-helix formation by assays of RNase H cleavage inhibition in vitro using two systems (two-strand-system (FTFOs) or three-strand-system (TFOs)) targeted to the polypurine tract (PPT) of HIV-1. The two-stranded composition of a triple-helix is thermodynamically and kinetically superior to the three-strand-system, The FTFOs inhibited the RNase H activity in a sequence-specific manner, i.e., the tripler actually formed at the PPT and blocked the RNase H. The FTFOs containing the phosphorothioate groups at the antisense strand showed greater 3'-exonuclease resistance. InHIV-1 infected MT-4 cells, the ETFOs containing the phosphorothioate groups at the antisense strand and guanosine rich parts within the third Hoogsteen base pairing sequence inhibit the replication of HIV-1 more effectively than the antisense phosphorothioate oligonucleotides, indicating sequence-specific inhibition of HIV-1 replication.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/09/20 alle ore 21:45:23