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Titolo:
Interference of biocytin with opioid-evoked hyperpolarization and membraneproperties of rat spinal substantia gelatinosa neurons
Autore:
Eckert, WA; Willcockson, HH; Light, AR;
Indirizzi:
Univ N Carolina, Dept Cell & Mol Physiol, Chapel Hill, NC 27599 USA Univ NCarolina Chapel Hill NC USA 27599 ysiol, Chapel Hill, NC 27599 USA
Titolo Testata:
NEUROSCIENCE LETTERS
fascicolo: 2, volume: 297, anno: 2001,
pagine: 117 - 120
SICI:
0304-3940(20010112)297:2<117:IOBWOH>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
NEUROBIOTIN;
Keywords:
techniques; methods; lamina II; opioid receptors; patch clamp; in vitro slice; spinal cord;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
9
Recensione:
Indirizzi per estratti:
Indirizzo: Light, AR Univ N Carolina, Dept Cell & Mol Physiol, CB 7545 Med Sci Res Bldg, ChapelHill, NC 27599 USA Univ N Carolina CB 7545 Med Sci Res Bldg Chapel Hill NC USA 27599
Citazione:
W.A. Eckert et al., "Interference of biocytin with opioid-evoked hyperpolarization and membraneproperties of rat spinal substantia gelatinosa neurons", NEUROSCI L, 297(2), 2001, pp. 117-120

Abstract

In our laboratory, preliminary whole-cell, tight seal recordings of rat spinal substantia gelatinosa neurons including biocytin in the patch pipette yielded a significantly smaller proportion of neurons hyperpolarized by selective opioid agonists compared with recordings without biocytin. Therefore, we investigated the effects of biocytin inclusion on opioid responses andother membrane properties during whole-cell, tight seal recordings of these neurons. The percen tage of neurons hyperpolarized by mu-, delta (1)-, and kappa -selective opioids was significantly reduced when 1% but not less than or equal to0.2% biocytin was included in the recording pipette, compared with neurons recorded without biocytin. However, a significantly higher proportion of neurons fired spontaneous action potentials with either 0.05-0.2 or 1% biocytin com pa red to no biocytin. Resting membrane potential, input impedance and the proportion of neurons displaying transient outward rectification were each significantly altered for neurons recorded with 1% but not 0.05-0.2% biocytin. These effects may be due to a relatively specificblockade of diverse potassium channel types. Because efficient labeling can be achieved with 0.1% biocytin with whole-cell recording, higher concentrations are cantraindicated. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 17:54:09