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Titolo:
Coordination of microtubules and the actin cytoskeleton by the Rho effector mDia1
Autore:
Ishizaki, T; Morishima, Y; Okamoto, M; Furuyashiki, T; Kato, T; Narumiya, S;
Indirizzi:
Kyoto Univ, Fac Med, Dept Pharmacol, Kyoto 6068501, Japan Kyoto Univ Kyoto Japan 6068501 Med, Dept Pharmacol, Kyoto 6068501, Japan
Titolo Testata:
NATURE CELL BIOLOGY
fascicolo: 1, volume: 3, anno: 2001,
pagine: 8 - 14
SICI:
1465-7392(200101)3:1<8:COMATA>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
BINDING PROTEIN-RHO; SWISS 3T3 FIBROBLASTS; FOCAL ADHESIONS; CELL-MIGRATION; STRESS FIBERS; SACCHAROMYCES-CEREVISIAE; ARP2/3 COMPLEX; FISSION YEAST; KINASE; POLARIZATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Ishizaki, T Kyoto Univ, Fac Med, Dept Pharmacol, Kyoto 6068501, Japan Kyoto Univ Kyoto Japan 6068501 armacol, Kyoto 6068501, Japan
Citazione:
T. Ishizaki et al., "Coordination of microtubules and the actin cytoskeleton by the Rho effector mDia1", NAT CELL BI, 3(1), 2001, pp. 8-14

Abstract

Coordination of microtubules and the actin cytoskeleton is important in several types of cell movement. mDia1 is a member of the formin-homology family of proteins and an effector of the small GTPase Rho. It contains the Rho-binding domain in its amino terminus and two distinct regions of formin homology, FH1 in the middle and FH2 in the carboxy terminus. Here we show that expression of mDia1(Delta N3), an active mDia1 mutant containing the FH1 and FH2 regions without the Rho-binding domain, induces bipolar elongation of HeLa cells and aligns microtubules in parallel to F-actin bundles along the long axis of the cell. The cell elongation and microtubule alignment caused by this mutant is abolished by co-expression of an FH2-region fragment, and expression of mDia1(Delta N3) containing point mutations in the FH2 region causes an increase in the amount of disorganized F-actin without cellelongation and microtubule alignment. These results indicate that mDia1 may coordinate microtubules and F-actin through its FH2 and FH1 regions, respectively.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 15:13:49