Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Characterization, fate, and function of hamster cortical granule components
Autore:
Hoodbhoy, T; Talbot, P;
Indirizzi:
Univ Calif Riverside, Dept Biol, Riverside, CA 92521 USA Univ Calif Riverside Riverside CA USA 92521 Biol, Riverside, CA 92521 USA Univ Calif Riverside, Dept Cell Biol & Neurosci, Riverside, CA 92521 USA Univ Calif Riverside Riverside CA USA 92521 osci, Riverside, CA 92521 USA
Titolo Testata:
MOLECULAR REPRODUCTION AND DEVELOPMENT
fascicolo: 2, volume: 58, anno: 2001,
pagine: 223 - 235
SICI:
1040-452X(200102)58:2<223:CFAFOH>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
SEA-URCHIN EMBRYO; MOUSE EGGS; EXTRACELLULAR-MATRIX; MEIOTIC MATURATION; ZONA-PELLUCIDA; SPERM PENETRATION; PERIVITELLINE SPACE; CELL-ADHESION; OOCYTES; FERTILIZATION;
Keywords:
cortical granules; mammals; oocyte; glycoconjugates; embryo; preimplantation development; cleavage;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Talbot, P Univ Calif Riverside, Dept Biol, Riverside, CA 92521 USA Univ Calif Riverside Riverside CA USA 92521 rside, CA 92521 USA
Citazione:
T. Hoodbhoy e P. Talbot, "Characterization, fate, and function of hamster cortical granule components", MOL REPROD, 58(2), 2001, pp. 223-235

Abstract

Little is known about the composition and function of mammalian cortical granules. In this study, lectins were used as tools to: (1) estimate the number and molecular weight of glycoconjugates in hamster cortical granules and show what sugars are associated with each glycoconjugate; (2) identify cortical granule components that remain associated with the oolemma, corticalgranule envelope, and/or zona pel lucida of fertilized oocytes a nd preimplantation embryos; and (3) examine the role of cortical granule glycoconjugates in preimplantation embryogenesis. Microscopic examination of unfertilized oocytes revealed that the lectins PNA, DBA, WGA, RCA(120), Con A, and LCA bound to hamster cortical granules. Moreover, LCA and Con A labeled the zona pellucida, cortical granule envelope, and plasma membrane of fertilized and artificially activated oocytes and two and eight cell embryos. Lectinblots of unfertilized oocytes had at least 12 glycoconjugates that were recognized by one or more lectins. Nine of these glycoconjugates are found inthe cortical granule envelope and/or are associated with the zona pellucida and plasma membrane following fertilization. In vivo functional studies showed that the binding of Con A to one or more mannosylated cortical granule components inhibited blastomere cleavage in two-cell embryos. Our data show that hamster cortical granules contain approximately 12 glycoconjugates of which nine remain associated extracellularly with the fertilized oocyte after the cortical reaction and that one or more play a role in regulating cleavage divisions. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 22:21:21