Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Heat-shock resistance in experimental cryptorchid testis of mice
Autore:
Kon, Y; Endoh, D;
Indirizzi:
Hokkaido Univ, Grad Sch Vet Med, Dept Dis Control, Lab Expt Anim Sci, Sapporo, Hokkaido 0600818, Japan Hokkaido Univ Sapporo Hokkaido Japan 0600818 oro, Hokkaido 0600818, Japan Rakuno Gakuen Univ, Fac Med Vet, Dept Radiat Biol, Ebetsu, Hokkaido, JapanRakuno Gakuen Univ Ebetsu Hokkaido Japan t Biol, Ebetsu, Hokkaido, Japan
Titolo Testata:
MOLECULAR REPRODUCTION AND DEVELOPMENT
fascicolo: 2, volume: 58, anno: 2001,
pagine: 216 - 222
SICI:
1040-452X(200102)58:2<216:HRIECT>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
GERM-CELL APOPTOSIS; MOUSE SPERMATOGENIC CELLS; TESTICULAR DESCENT; PROTEIN; HSP70-2; GENE; SPERMATOCYTES; RAT;
Keywords:
cryptorchidism; heat-shock; mouse; MRL/MpJ; testis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Kon, Y Hokkaido Univ, Grad Sch Vet Med, Dept Dis Control, Lab Expt Anim Sci, Sapporo, Hokkaido 0600818, Japan Hokkaido Univ Sapporo Hokkaido Japan 0600818 kkaido 0600818, Japan
Citazione:
Y. Kon e D. Endoh, "Heat-shock resistance in experimental cryptorchid testis of mice", MOL REPROD, 58(2), 2001, pp. 216-222

Abstract

Cryptorchidism is commonly used for research on spermatogenesis. However, there are few comparative investigations about the strain differences in mice, especially in long-term experiments. In the present study, the authors demonstrate its specific dynamics in the MRL/MpJ mouse strain, and discuss the cause of strain differences. In the mouse strains A/J BALB/c, C3H/He, and C57BL/6, after 2 weeks of experimental cryptorchidism, the ratios of thecryptorchid testis weight against; the intact one were 0.38 +/- 0.05, 0.43+/- 0.05, 0.38 +/- 0.02, and 0.44 +/- 0.14, respectively. On the other hand, in the MRL/MpJ strain it was shifted to 0.69 +/- 0.08. The details of this strain difference were compared by calculation of germ cells with the Sertoli cell index at 2 weeks after operation. The indices of spermatogonia in all strains were not significantly different; however, in MRL/MpJ mice remarkable numbers of late spermatocytes and round spermatids were detected. The decrease of the testis weight ratio was similar until 10 days in the C57BL/6 and MRL/MpJ strains, but continued in C57BL/6 until 21 days, whereas in MRL/MpJ mice it plateaued after 10 days. Northern blot analysis for heatshock protein 70-2 using total RNA prepared from the cryptorchid and intact testes at 2 weeks after operation revealed that the expression was decreased in the cryptorchid testis of C57BL/6, but not MRL/MpJ mice. The resultssuggested that heat-resistant germ cells were present in MRL/MpJ, originating possibly from the genetic background. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 01:26:06