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Titolo:
Apparent absence of oct 3/4 from the chicken genome
Autore:
Soodeen-Karamath, S; Gibbins, AMV;
Indirizzi:
Univ Guelph, Dept Anim & Poultry Sci, Guelph, ON N1G 2W1, Canada Univ Guelph Guelph ON Canada N1G 2W1 ltry Sci, Guelph, ON N1G 2W1, Canada
Titolo Testata:
MOLECULAR REPRODUCTION AND DEVELOPMENT
fascicolo: 2, volume: 58, anno: 2001,
pagine: 137 - 148
SICI:
1040-452X(200102)58:2<137:AAOO3F>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
PRIMORDIAL GERM-CELLS; LEUKEMIA INHIBITORY FACTOR; EMBRYONIC STEM-CELLS; POU-SPECIFIC DOMAIN; TRANSCRIPTION FACTOR; DNA-BINDING; CHROMOSOMAL LOCATION; BLASTODERMAL CELLS; XENOPUS EMBRYOS; EXPRESSION;
Keywords:
POU; PGC; ES cells; avian;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
61
Recensione:
Indirizzi per estratti:
Indirizzo: Gibbins, AMV Univ Guelph, Dept Anim & Poultry Sci, Guelph, ON N1G 2W1, Canada Univ Guelph Guelph ON Canada N1G 2W1 lph, ON N1G 2W1, Canada
Citazione:
S. Soodeen-Karamath e A.M.V. Gibbins, "Apparent absence of oct 3/4 from the chicken genome", MOL REPROD, 58(2), 2001, pp. 137-148

Abstract

The development of efficient methods for establishing germline competent chicken embryonic stem (ES) cell lines has proved elusive. In the mouse embryo, expression of oct 3/4 is limited to pluripotent cells and primordial germ cells; regulatory sequences of this gene have been used to derive germline competent mouse ES cell lines by the continuous ablation of differentiated cells in culture using drug selection. To apply this technique to chickens several strategies were employed to analyze the chicken genome for oct 3/4, a member of the highly conserved POU gene family. PCR and Southern hybridization experiments with primers and probes based on mouse oct 3/4 sequences indicated that oct 3/4-like sequences are not present in the chicken genome. Also, analysis of mRNA from Stage 14 and 20 (H&H) chick embryos by reverse transcription PCR and the screening of a Stage 20 (H&H) chick embryo cDNA library with mouse oct 3/4-based primers and probes indicated that oct3/4-like sequences are not expressed in the early chick embryo. The apparent absence of oct 3/4 in chickens, despite the conservation of the gene in mammals and urodeles, is discussed in terms of possible implications for the mode of chicken PGC formation in relation to that in other vertebrates, (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 16:26:37