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Titolo:
Formation and characterization of planar phospholipid bilayers supported on TiO2 and SrTiO3 single crystals
Autore:
Starr, TE; Thompson, NL;
Indirizzi:
Univ N Carolina, Dept Chem, Chapel Hill, NC 27599 USA Univ N Carolina Chapel Hill NC USA 27599 Chem, Chapel Hill, NC 27599 USA
Titolo Testata:
LANGMUIR
fascicolo: 26, volume: 16, anno: 2000,
pagine: 10301 - 10308
SICI:
0743-7463(200012)16:26<10301:FACOPP>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
ATOMIC-FORCE MICROSCOPY; INTERNAL-REFLECTION FLUORESCENCE; SCANNING TUNNELING MICROSCOPY; POLYMER-CUSHIONED BILAYERS; METAL-OXIDE SURFACES; LIPID BILAYERS; LATERAL DIFFUSION; SOLID SUPPORTS; MEMBRANES; MONOLAYERS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
74
Recensione:
Indirizzi per estratti:
Indirizzo: Thompson, NL Univ N Carolina, Dept Chem, Campsu Box 3290, Chapel Hill, NC 27599 USA Univ N Carolina Campsu Box 3290 Chapel Hill NC USA 27599 USA
Citazione:
T.E. Starr e N.L. Thompson, "Formation and characterization of planar phospholipid bilayers supported on TiO2 and SrTiO3 single crystals", LANGMUIR, 16(26), 2000, pp. 10301-10308

Abstract

Supported phospholipid bilayers have been prepared on rutile TiO2 and SrTiO3 single-crystal substrates. Fluidlike bilayers were successfully deposited onto each surface by Langmuir-Blodgett and Langmuir-Schaeffer methods andthe vesicle adsorption and fusion method. The characteristics of membranessupported on TiO2 and SrTiO3 single crystals were evaluated using fluorescence pattern photobleaching recovery, fluorescence imaging, and differential interference contrast imaging, and the results were compared to similar preparations on fused silica. Diffusion coefficients of a fluorescent lipid in membranes supported on all substrates;were on the order of 10(-8) cm(2)/s, and fractional mobilities were 74-99%: Measured diffusion coefficients were faster for fluorescent lipids in bilayers on TiO2 and SrTiO3 than in bilayers on fused silica, and some sample types exhibited two diffusing species rather than a single diffusing species. Both the intensities and bleachabilities of the fluorescent lipids were significantly reduced for bilayers on TiO2 and SrTiO3 as compared to fused silica. A new theoretical model forinterpreting fluorescence pattern photobleaching recovery data for fluorescent lipid diffusion in planar membranes is described. The ability to form planar phospholipid bilayers on TiO2 and SrTiO3 is expected to facilitate new developments in total internal reflection fluorescence microscopy, surface-based assays of importance in biotechnology, and biosensors.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 18:27:57