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Titolo:
Sulfate homeostasis, NaSi-1 cotransporter, and SAT-1 exchanger expression in chronic renal failure in rats
Autore:
Fernandes, I; Laouari, D; Tutt, P; Hampson, G; Friedlander, G; Silve, C;
Indirizzi:
Fac Med Xavier Bichat, INSERM, U426, F-75018 Paris, France Fac Med Xavier Bichat Paris France F-75018 , U426, F-75018 Paris, France Fac Med Xavier Bichat, Inst Federat Rech Cellules Epitheliales, F-75018 Paris, France Fac Med Xavier Bichat Paris France F-75018 liales, F-75018 Paris, France St Thomas Hosp, Dept Chem Pathol, London, England St Thomas Hosp London England s Hosp, Dept Chem Pathol, London, England
Titolo Testata:
KIDNEY INTERNATIONAL
fascicolo: 1, volume: 59, anno: 2001,
pagine: 210 - 221
SICI:
0085-2538(200101)59:1<210:SHNCAS>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
ALTERED DIETARY SULFATE; NA-K-ATPASE; INORGANIC SULFATE; CYTOSOLIC SULFOTRANSFERASES; PARATHYROID-HORMONE; I COTRANSPORTER; MESSENGER-RNA; PROTEIN DIETS; KIDNEY; TRANSPORT;
Keywords:
proximal tubule; fractional sulfate excretion; hypersulfatemia; hyperphosphatemia; renal impairment; kidney osteodystrophy; transport; phosphate homeostasis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
55
Recensione:
Indirizzi per estratti:
Indirizzo: Silve, C Fac Med Xavier Bichat, INSERM, U426, 16 Rue Henri Huchard, F-75018 Paris, France Fac Med Xavier Bichat 16 Rue Henri Huchard Paris France F-75018
Citazione:
I. Fernandes et al., "Sulfate homeostasis, NaSi-1 cotransporter, and SAT-1 exchanger expression in chronic renal failure in rats", KIDNEY INT, 59(1), 2001, pp. 210-221

Abstract

Background. It is known that hypersulfatemia, like hyperphosphatemia, occurs in chronic renal failure (CRF). The aim of this study was to assess the effects of CRF on sulfate homeostasis and on sodium sulfate cotransport (NaSi-1) and sulfate/ oxalate-bicarbonate exchanger (Sat-1) expression in the kidney. In addition, sulfate homeostasis was compared with phosphate homeostasis. Methods. Experimental studies were performed in adult male rats at three and six weeks after 80% subtotal nephrectomy (Nx) or sham-operation (8) (N =9 per group). Transporter protein and mRNA expressions were measured by Western blot and RNase protection assay (RPA), respectively. Results were quantitated by densitometric scanning (Western) and electronic autoradiography (RPA), and were expressed in densitometric units (DUs: Western) and cpm (RPA). Results. Creatinine clearance was lower in Nx-3 compared with S-3 rats (0.23 vs. 0.51 mL/min/100 g body weight, P < 0.001) and was further impaired in Nx-6 rats (0.15 vs. 0.48, P < 0.001). Sulfatemia wassignificantly higher in Nx-3 rats (1.08 vs. 0.84 mmol/L, P < 0.05) and further increased in Nx-6 rats (1.42 vs. 0.90 mmol/L, P < 0.01). Fractional sulfate excretion (FESO4) was increased by twofold in Nx-3 and Nx-6 rats compared with corresponding S rats. Phosphatemia did not differ between Nx-3 rats and controls, but was increased in Nx-6 rats (P < 0.01). Total amounts of both NaSi-1 and Sat-1 proteins were significantly decreased in both Nx-3 and Nx-6 rats when compared with controls. However, NaSi-1 protein and mRNAdensities did not significantly change in Nx-3 rats, but were significantly increased in Nx-6 rats when compared with controls (4.8 vs. 3.7 DU/<mu>g protein, P < 0.05, and 7.1 vs. 2.8 cpm/<mu>g RNA, P < 0.01, respectively, for protein and mRNA). In contrast to NaSi-1, Sat-1 protein density was significantly decreased both in Nx-3 (2.9 vs. 3.6 DU/<mu>g protein, P < 0.05) and Nx-6 rats (2.4 vs. 3.4 DU/<mu>g protein, P < 0.05), and Sat-1 mRNA density significantly decreased in Nx-6 rats (10.7 vs. 14.7 cpm/<mu>g RNA, P < 0.05). Na-PO4 cotransporter (NaPi-2) protein total abundance and density were decreased at three and six weeks in Nx rats. Conclusions. These results demonstrate that both NaSi-1 and Sat-1 total protein abundances are decreased in CRF, which may contribute to the increasein fractional sulfate excretion. Strikingly, NaSi-1 density was not decreased in CRF three weeks after Nx, and furthermore, increased six weeks afterNx, in contrast to NaPi-2 density, which was decreased at both times. The significance of this difference remains to be determined, but may explain why hypersulfatemia occurs earlier than hyperphosphatemia in CRF.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/07/20 alle ore 01:41:36