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Titolo:
Characteristics of sustained blood-brain barrier opening and tissue injuryin a model for focal trauma in the rat
Autore:
Preston, E; Webster, J; Small, D;
Indirizzi:
Natl Res Council Canada, Inst Biol Sci, Ottawa, ON K1A 0R6, Canada Natl Res Council Canada Ottawa ON Canada K1A 0R6 tawa, ON K1A 0R6, Canada
Titolo Testata:
JOURNAL OF NEUROTRAUMA
fascicolo: 1, volume: 18, anno: 2001,
pagine: 83 - 92
SICI:
0897-7151(200101)18:1<83:COSBBO>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTRACEREBRAL HEMORRHAGE; FOREBRAIN ISCHEMIA; TRANSFER CONSTANTS; EDEMA FORMATION; S-100 PROTEIN; PERMEABILITY; MECHANISMS; ASTROCYTES; TRANSPORT; CELL;
Keywords:
blood-brain barrier; brain edema; brain trauma; intracranial hemorrhage;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Preston, E Natl Res Council Canada, Inst Biol Sci, M54 Montreal Rd, Ottawa, ON K1A 0R6, Canada Natl Res Council Canada M54 Montreal Rd Ottawa ON Canada K1A 0R6
Citazione:
E. Preston et al., "Characteristics of sustained blood-brain barrier opening and tissue injuryin a model for focal trauma in the rat", J NEUROTRAU, 18(1), 2001, pp. 83-92

Abstract

Minor stab wounding of rodent brain by needle or razor blade is a standardmodel for immunohistochemical investigations of secondary neuronal degeneration and scarring. Opening of the blood-brain barrier (BBB) to plasma molecules and inflammatory cells is integral to the secondary injury process. To facilitate quantitative study of these BBB phenomena, we tested the utility of a stereotaxic wire knife as a minimally invasive way for modeling of focal trauma and bleeding in brain parenchyma and substantial, reproducibleBBB damage. Adult rats were anesthetized, and through a skull burr hole, the 0.3-mm dia guide cannula housing a laterally extendable tungsten wire (0.13 mm dia) was inserted into the right striatum. A layering of horizontal disk-like cuts (3 mm dia) was made, producing a cylindrical lesion of similar to 18 mm(3) volume, similar to2.7% of the cerebral hemisphere. Transfer constants (K-i) for blood to brain permeation of [H-3]sucrose measured from30 min to 2 weeks postlesion showed sustained BBB leakiness; for example, mean K-i +/- SEM (nL.g(-1).s(-1)) for a standard, matrix-dissected forebrain sample enclosing the lesion were 7.2 +/- 1.2 (day 1 postlesion), 8.1 +/- 1.4 (day 3), 5.4 +/- 0.8 (day 14) compared with values for contralateral nonlesioned forebrain ranging from 1.3 +/- 0.05 to 1.6 +/- 0.3 (n = 3-4 samples per time point). Analysis of the simultaneous transport of [C-14]sucrose(MW = 342 Da) and [H-3]inulin (MW similar to5,000) showed significantly larger upward increments in K-i for sucrose than inulin, indicating a pore-like opening mechanism. Significant edema was measured 3 days postlesion. A reactive glial response was indicated by an increase in S100 beta by 6 h anda glial scar forming around the lesion by 7 days. Secondary brain injury was indicated by a 10% loss of hemisphere mass, measured at 2 months. The wire knife enabled tailoring of interstitial trauma with a minimum of extraneous injury and supported reproducible measurements of sustained BBB injury using relatively few animals.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/07/20 alle ore 17:34:23