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Titolo:
Targeting murine immune responses to selected T cell- or antibody-defined determinants of the hepatitis B surface antigen by plasmid DNA vaccines encoding chimeric antigen
Autore:
Schirmbeck, R; Zheng, X; Roggendorf, M; Geissler, M; Chisari, FV; Reimann, J; Lu, MJ;
Indirizzi:
Univ Ulm, Inst Med Microbiol & Immunol, D-89081 Ulm, Germany Univ Ulm Ulm Germany D-89081 d Microbiol & Immunol, D-89081 Ulm, Germany Univ Essen, Inst Virol, Essen, Germany Univ Essen Essen GermanyUniv Essen, Inst Virol, Essen, Germany Univ Hosp Freiburg, Dept Internal Med 2, Freiburg, Germany Univ Hosp Freiburg Freiburg Germany t Internal Med 2, Freiburg, Germany Scripps Clin & Res Inst, Div Expt Pathol, La Jolla, CA 92037 USA Scripps Clin & Res Inst La Jolla CA USA 92037 hol, La Jolla, CA 92037 USA
Titolo Testata:
JOURNAL OF IMMUNOLOGY
fascicolo: 2, volume: 166, anno: 2001,
pagine: 1405 - 1413
SICI:
0022-1767(20010115)166:2<1405:TMIRTS>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENDOGENOUS PROTEIN ANTIGENS; VIRUS TRANSGENIC MICE; HANSENULA-POLYMORPHA; LYMPHOCYTE RESPONSES; IMMUNOGENICITY; PARTICLES; YEAST; PATHOGENESIS; IMMUNIZATION; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Schirmbeck, R Univ Ulm, Inst Med Microbiol & Immunol, Albert Einstein Allee 11, D-89081 Ulm, Germany Univ Ulm Albert Einstein Allee 11 Ulm Germany D-89081 rmany
Citazione:
R. Schirmbeck et al., "Targeting murine immune responses to selected T cell- or antibody-defined determinants of the hepatitis B surface antigen by plasmid DNA vaccines encoding chimeric antigen", J IMMUNOL, 166(2), 2001, pp. 1405-1413

Abstract

By exchanging sequences from the middle-surface (MS) and small-surface (S)Ag of hepatitis B virus (HBV) with corresponding sequences of the MS Ag ofwoodchuck hepatitis virus, we constructed chimeric MS variants. Using these constructs as DNA vaccines in mice, we selectively primed highly specific(non-cross-reactive) Ab responses to pre-S2 of the HBV MS Ag and the "a" determinant of the HBV S Ag, as well as L-d- or K-b-restricted CTL responsesto HBV S epitopes. In transgenic mice that constitutively express large amounts of HBV surface Ag in the liver we could successfully suppress serum antigenemia (but not Ag production in the liver) by adoptive transfer of anti-pre-S2 or anti-"a" immunity but not CTL immunity. DNA vaccines greatly facilitate construction of chimeric fusion Ags that efficiently prime specific, high-affinity Ab and CTL responses. Such vaccines, in which sequences ofan Ag of interest are exchanged between different but related viruses, areinteresting tools for focusing humoral or cellular immunity on selected antigenic determinants and elucidating their biological role.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/09/20 alle ore 21:49:49