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Titolo:
Prostaglandin E-2 suppressed IL-15-mediated human NK cell function throughdown-regulation of common gamma-chain
Autore:
Joshi, PC; Zhou, XC; Cuchens, M; Jones, Q;
Indirizzi:
Univ Mississippi, Med Ctr, Dept Surg, Jackson, MS 39216 USA Univ Mississippi Jackson MS USA 39216 r, Dept Surg, Jackson, MS 39216 USA Univ Mississippi, Med Ctr, Dept Microbiol, Jackson, MS 39216 USA Univ Mississippi Jackson MS USA 39216 pt Microbiol, Jackson, MS 39216 USA
Titolo Testata:
JOURNAL OF IMMUNOLOGY
fascicolo: 2, volume: 166, anno: 2001,
pagine: 885 - 891
SICI:
0022-1767(20010115)166:2<885:PESIHN>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
NATURAL-KILLER-CELLS; IL-2 RECEPTOR; T-CELLS; GROWTH-FACTOR; EXPRESSION; INTERLEUKIN-2; CYTOKINE; LYMPHOCYTES; GENERATION; MONOCYTES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Joshi, PC Univ Mississippi, Med Ctr, Dept Surg, 2500 N State St, Jackson, MS 39216 USA Univ Mississippi 2500 N State St Jackson MS USA 39216 39216 USA
Citazione:
P.C. Joshi et al., "Prostaglandin E-2 suppressed IL-15-mediated human NK cell function throughdown-regulation of common gamma-chain", J IMMUNOL, 166(2), 2001, pp. 885-891

Abstract

NK cell function is regulated by cytokines and certain biochemical mediators in a positive or negative manner. This study was performed to investigate the suppressive effects of PGE(2) on IL-15-activated human NK cell function. Purified NK cells were cultured with 200 ng/ml IL-15 for 2 days in the presence or absence of 10-200 ng/ml PGE(2), PGE(2) significantly suppressedNK cell-mediated cytotoxicity and IFN-gamma production at the secretional and the transcriptional levels. We also evaluated the effect of PGE(2) on the IL-15R complex that consists of IL-2R beta, common gamma -chain (gamma (c)-chain), and a specific chain IL-15 alpha. Percentage of positive cells and number of binding sites for gamma (c)-chain were significantly increasedafter IL-15 treatment; however, a substantial decrease was observed with PGE(2) cotreatment. In contrast, constitutive expression of IL-2R beta was significantly decreased after IL-15 treatment, with no change detected in the presence of PGE(2). At the transcriptional level, neither IL-15 nor PGE(2) had significant effects on the expression of beta- or gamma (c)-chains. There was a 3-fold increase in the expression of IL-15 alpha at the transcriptional level that peaked at 8 h after IL-15 treatment; however, PGE(2) hadno significant effect. Suppression of NK function by PGE(2) was not due tothe endogenous production of IL-4, IL-10, or TGF-beta (1) by NK cells. These results suggest that down-regulation of surface expression of gamma (c)-chain on NK cells may be one mechanism through which PGE(2) mediates suppression of IL-15-activated NK cell function.

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Documento generato il 30/03/20 alle ore 00:43:24