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Titolo:
RNAs 1 and 2 of Alfalfa mosaic virus, expressed in transgenic plants, start to replicate only after infection of the plants with RNA 3
Autore:
Thole, V; Garcia, ML; van Rossum, CMA; Neeleman, L; Brederode, FT; Linthorst, HJM; Bol, JF;
Indirizzi:
Leiden Univ, Gorlaeus Labs, Inst Mol Plant Sci, NL-2300 RA Leiden, Netherlands Leiden Univ Leiden Netherlands NL-2300 RA NL-2300 RA Leiden, Netherlands
Titolo Testata:
JOURNAL OF GENERAL VIROLOGY
, volume: 82, anno: 2001,
parte:, 1
pagine: 25 - 28
SICI:
0022-1317(200101)82:<25:R1A2OA>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
COAT PROTEIN; TOBACCO PLANTS; IN-VITRO; GENES; AMPLIFICATION; TRANSLATION; INITIATION; PLUS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Bol, JF Leiden Univ, Gorlaeus Labs, Inst Mol Plant Sci, POB 9502, NL-2300 RA Leiden, Netherlands Leiden Univ POB 9502 Leiden Netherlands NL-2300 RA n, Netherlands
Citazione:
V. Thole et al., "RNAs 1 and 2 of Alfalfa mosaic virus, expressed in transgenic plants, start to replicate only after infection of the plants with RNA 3", J GEN VIROL, 82, 2001, pp. 25-28

Abstract

RNAs 1 and 2 of the tripartite genome of Alfalfa mosaic virus (AMV) encodethe two viral replicase subunits. Full-length DNA copies of RNAs 1 and 2 were used to transform tobacco plants (R12 lines). None of the transgenic lines showed resistance to AMV infection. In healthy R12 plants, the transcripts of the viral cDNAs were copied by the transgenic viral replicase into minus-strand RNAs but subsequent steps in replication were blocked. When theR12 plants were inoculated with AMV RNA 3, this block was lifted and the transgenic RNAs 1 and 2 were amplified by the transgenic replicase together with RNA 3. The transgenic expression of RNAs 1 and 2 largely circumvented the role of coat protein (CP) in the inoculum that is required for infection of nontransgenic plants. The results for the first time demonstrate the role of CP in AMV plus-strand RNA synthesis at the whole plant level.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 21:42:03