Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Identification of macrophage migration inhibitory factor as a potent endothelial cell growth-promoting agent released by ectopic human endometrial cells
Autore:
Yang, YB; Degranpre, P; Kharfi, A; Akoum, A;
Indirizzi:
Univ Laval, Ctr Hosp Univ Quebec, Ctr Rech, Lab Endocrinol Reprod, Quebec City, PQ G1L 3L5, Canada Univ Laval Quebec City PQ Canada G1L 3L5 Quebec City, PQ G1L 3L5, Canada
Titolo Testata:
JOURNAL OF CLINICAL ENDOCRINOLOGY AND METABOLISM
fascicolo: 12, volume: 85, anno: 2000,
pagine: 4721 - 4727
SICI:
0021-972X(200012)85:12<4721:IOMMIF>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
MONOCYTE CHEMOTACTIC PROTEIN-1; PERITONEAL-FLUID; FACTOR MIF; IMMUNOHISTOCHEMICAL LOCALIZATION; INFERTILE WOMEN; IN-VITRO; ANGIOGENESIS; EXPRESSION; RAT; PATHOGENESIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
53
Recensione:
Indirizzi per estratti:
Indirizzo: Akoum, A Hop St Francois Assise, Ctr Rech, Lab Endocrinol Reprod, 10 Rue Espinay,Local D0-711, Quebec City, PQ G1L 3L5, Canada Hop St Francois Assise10 Rue Espinay,Local D0-711 Quebec City PQ Canada G1L 3L5
Citazione:
Y.B. Yang et al., "Identification of macrophage migration inhibitory factor as a potent endothelial cell growth-promoting agent released by ectopic human endometrial cells", J CLIN END, 85(12), 2000, pp. 4721-4727

Abstract

The growth of endometrial cells in ectopic locations (endometriosis) is dependent on the establishment of an adequate blood supply. Neovascularization (angiogenesis) is therefore a vital step toward the progression of this disease. We first revealed the presence of a potent mitogenic activity for human endothelial cells in the culture medium of immortalized human endometriotic cells. The activity, measured by the level of [H-3]-thymidine incorporation into the DNA of human coronary artery endothelial cells, was then purified by anion exchange high-performance liquid chromatography. Electrophoretic analysis of one of the bioactive fractions that markedly enhanced endothelial cell proliferation showed three distinct bands with apparent molecular masses of 15.8, 12.6, and 6.5 kDa. N-terminal microsequencing of an internal peptide from the 12.6-kDa protein showed 100% homology with human macrophage migration inhibitory factor (MIF). The protein was positively identified as MIF by Western blot analysis using a specific anti-MIF antibody. Anti-MIF antibody inhibited the bioactivity found in the evaluated fractionand the conditioned medium of primary endometriotic cell cultures, and commercial recombinant human MIF displayed a high mitogenic activity for endothelial cells. Our findings reveal that MIF is released by endometriotic cells and acts as a potent mitogenic factor for human endothelial cells in vitro. This may have a considerable interest, in view of the crucial role of angiogenesis in ectopic endometrial cell growth and activity and in numeroustissues undergoing dynamic physiological changes, such as human endometrium.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 12:12:08