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Titolo:
A sensitive high-performance liquid chromatographic method using electrochemical detection for the analysis of olanzapine and desmethylolanzapine in plasma of schizophrenic patients using a new solid-phase extraction procedure
Autore:
Raggi, MA; Casamenti, G; Mandrioli, R; Volterra, V;
Indirizzi:
Univ Bologna, Dept Pharmaceut Sci, I-40126 Bologna, Italy Univ Bologna Bologna Italy I-40126 harmaceut Sci, I-40126 Bologna, Italy Univ Bologna, Psychiat Clin, I-40123 Bologna, Italy Univ Bologna BolognaItaly I-40123 Psychiat Clin, I-40123 Bologna, Italy
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 1, volume: 750, anno: 2001,
pagine: 137 - 146
SICI:
1387-2273(20010105)750:1<137:ASHLCM>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
ANTIPSYCHOTIC AGENT OLANZAPINE; MASS-SPECTROMETRY; DOUBLE-BLIND; SERUM; METABOLITES; PLACEBO; TRIAL;
Keywords:
olanzapine; desmethylolanzapine; liquid chromatography; amperometric detection; solid-phase extraction; human plasma;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Raggi, MA Univ Bologna, Dept Pharmaceut Sci, Via Belmeloro 6, I-40126 Bologna, Italy Univ Bologna Via Belmeloro 6 Bologna Italy I-40126 logna, Italy
Citazione:
M.A. Raggi et al., "A sensitive high-performance liquid chromatographic method using electrochemical detection for the analysis of olanzapine and desmethylolanzapine in plasma of schizophrenic patients using a new solid-phase extraction procedure", J CHROMAT B, 750(1), 2001, pp. 137-146

Abstract

A high-performance liquid chromatographic method with amperometric detection for the analysis of the novel antipsychotic drug olanzapine and its metabolite desmethylolanzapine in human plasma has been developed. The analysiswas carried out on a reversed-phase column (C-8, 150x4.6 mm I.D., 5 mum) using acetonitrile-phosphate buffer, pH 3.8, as the mobile phase. The detection voltage was +800 mV and the cell and column temperature was 30 degreesC. The flow-rate was 1.2 ml min(-1). Linear responses were obtained between 5 and 150 ng ml(-1), with repeatability <3.3%. A careful pretreatment of the biological samples was implemented by means of solid-phase extraction (SPE) on C-8 cartridges. The method requires 500 <mu>l of plasma for one complete analysis. Absolute recovery exceeded 97% for both olanzapine and desmethylolanzapine, and the detection limit was 1 ng ml(-1) for both analytes. Repeatability, intermediate precision and accuracy were satisfactory. This sensitive and selective method has been successfully applied to therapeutic drug monitoring in schizophrenic patients treated with Zyprexa(R) tablets. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 17/01/20 alle ore 20:56:07