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Titolo:
Detection of a mixed-backbone oligonucleotide (GEM 231) in liver and tumortissues by capillary electrophoresis
Autore:
Bansal, R; Chen, HX; Marshall, JL; Tan, J; Glazer, RI; Wainer, IW;
Indirizzi:
Georgetown Univ, Med Ctr, Dept Pharmacol, Bioanalyt Ctr, Washington, DC 20007 USA Georgetown Univ Washington DC USA 20007 lyt Ctr, Washington, DC 20007 USA Georgetown Univ, Med Ctr, Lombardi Canc Ctr, Washington, DC 20007 USA Georgetown Univ Washington DC USA 20007 anc Ctr, Washington, DC 20007 USA
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 1, volume: 750, anno: 2001,
pagine: 129 - 135
SICI:
1387-2273(20010105)750:1<129:DOAMO
Fonte:
ISI
Lingua:
ENG
Soggetto:
ANION-EXCHANGE CHROMATOGRAPHY; PHOSPHOROTHIOATE OLIGONUCLEOTIDES; ANTISENSE OLIGONUCLEOTIDES; BIOLOGICAL-FLUIDS; QUANTITATIVE-ANALYSIS; IN-VIVO; METABOLISM; COMPLEMENT; KINETICS; SAMPLES;
Keywords:
GEM 231; oligonucleotides;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Wainer, IW Georgetown Univ, Med Ctr, Dept Pharmacol, Bioanalyt Ctr, 3900 Reservoir RdNW, Washington, DC 20007 USA Georgetown Univ 3900 Reservoir Rd NW Washington DC USA 20007 SA
Citazione:
R. Bansal et al., "Detection of a mixed-backbone oligonucleotide (GEM 231) in liver and tumortissues by capillary electrophoresis", J CHROMAT B, 750(1), 2001, pp. 129-135

Abstract

A simple, rapid and sensitive method has been developed and validated for the analysis of a mixed-backbone oligonucleotide (GEM 231) in tumor tissues. The analysis was performed using a capillary electrophoresis (CE) system with UV detection. An extended light path (bubble cell) capillary column of64.5 cm (effective length 56 cm)x50 mum I.D. is used as the separation column. The optimized chromatographic conditions were background electrolyte: sodium berate buffer (60 mM, pH 9.1), electrokinetic injection: 10 s, applied voltage: 30 kV, detection at lambda =210 nm. A linear relationship was observed between the peak area and the amount of GEM 231 in the range of 1.0-1000 mug/ml. The lower detection limit of the drug was 100 pg with an average recovery of about 75+/-5%. The inter-day and intra-day relative standard deviations were <10%. Assay validation studies revealed that CE method isreproducible and specific for the determination of GEM 231 in tissue homogenates with a run time of less than 5 min. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 01:10:45