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Titolo:
Simple and sensitive high-performance liquid chromatographic method for the determination of 1,5-benzodiazepine clobazam and its active metabolite N-desmethylclobazam in human serum and urine with application to 1,4-benzodiazepines analysis
Autore:
Kunicki, PK;
Indirizzi:
Natl Inst Cardiol, Dept Clin Biochem, Clin Pharmacol Unit, PL-04628 Warsaw, Poland Natl Inst Cardiol Warsaw Poland PL-04628 l Unit, PL-04628 Warsaw, Poland
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 1, volume: 750, anno: 2001,
pagine: 41 - 49
SICI:
1387-2273(20010105)750:1<41:SASHLC>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
SOLID-PHASE EXTRACTION; GAS-CHROMATOGRAPHY; ELECTRON-CAPTURE; HUMAN-PLASMA; BENZODIAZEPINES; QUANTITATION; HPLC; IDENTIFICATION; NORCLOBAZAM; NITRAZEPAM;
Keywords:
clobazam; benzodiazepines;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Kunicki, PK Natl Inst Cardiol, Dept Clin Biochem, Clin Pharmacol Unit, Alpejska 42, PL-04628 Warsaw, Poland Natl Inst Cardiol Alpejska 42 Warsaw Poland PL-04628 , Poland
Citazione:
P.K. Kunicki, "Simple and sensitive high-performance liquid chromatographic method for the determination of 1,5-benzodiazepine clobazam and its active metabolite N-desmethylclobazam in human serum and urine with application to 1,4-benzodiazepines analysis", J CHROMAT B, 750(1), 2001, pp. 41-49

Abstract

A HPLC-UV determination of clobazam and N-desmethylclobazam in human serumand urine is presented. After simple liquid-liquid extraction with dichloromethane the compounds and an internal standard diazepam were separated on a Supelcosil LC-8-DB column at ambient temperature under isocratic conditions using the mobile phase: CH3CN-water-0.5 M KH2PO4-H3PO4 (440:540:20:0.4, v/v and 360:580:60:0.4, v/v for serum and urine, respectively). The detection was performed at 228 nm with limits of quantification of 2 ng/ml for serum and 1 ng/ml for urine. Relative standard deviations for intra- and inter-assay precision were found below 8% for both compounds for all the tested concentrations. The described procedure may be easily adapted for several 1,4-benzodiazepines. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/01/20 alle ore 13:01:56