Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Characterization and reconstitution of Drosophila gamma-tubulin ring complex subunits
Autore:
Gunawardane, RN; Martin, OC; Cao, K; Zhang, LJ; Dej, K; Iwamatsu, A; Zheng, YX;
Indirizzi:
Carnegie Inst Washington, Howard Hughes Med Inst, Baltimore, MD 21210 USA Carnegie Inst Washington Baltimore MD USA 21210 , Baltimore, MD 21210 USA Johns Hopkins Univ, Dept Biol, Baltimore, MD 21218 USA Johns Hopkins UnivBaltimore MD USA 21218 t Biol, Baltimore, MD 21218 USA Kirin Brewery Co Ltd, Cent Labs Key Technol, Yokohama, Kanagawa, Japan Kirin Brewery Co Ltd Yokohama Kanagawa Japan , Yokohama, Kanagawa, Japan
Titolo Testata:
JOURNAL OF CELL BIOLOGY
fascicolo: 7, volume: 151, anno: 2000,
pagine: 1513 - 1523
SICI:
0021-9525(200012)151:7<1513:CARODG>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
SPINDLE POLE BODY; MICROTUBULE NUCLEATION; IN-VITRO; CENTROSOME; PROTEIN; SPC98P; RECRUITMENT; BINDING; MINUS;
Keywords:
centrosome; microtubule; gamma-tubulin; grip; Drosophila;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Gunawardane, RN Carnegie Inst Washington, Howard Hughes Med Inst, 115 W Univ Pkwy, Baltimore, MD 21210 USA Carnegie Inst Washington 115 W Univ Pkwy Baltimore MD USA 21210
Citazione:
R.N. Gunawardane et al., "Characterization and reconstitution of Drosophila gamma-tubulin ring complex subunits", J CELL BIOL, 151(7), 2000, pp. 1513-1523

Abstract

The gamma -tubulin ring complex (gamma TuRC) is important for microtubule nucleation from the centrosome. In addition to gamma -tubulin, the Drosophila gamma TuRC contains at least six subunits, three of which [Drosophila gamma ring proteins (Dgrips) 75/d75p, 84, and 91] have been characterized previously Dgrips84 and 91 are present in both the small gamma -tubulin complex (gamma TuSC) and the gamma TuRC, while the remaining subunits are found only in the gamma TuRC. To study gamma TuRC assembly and function, we first reconstituted gamma TuSC using the baculovirus expression system. Using thereconstituted gamma TuSC, we showed for the first time that this subcomplex of the gamma TuRC has microtubule binding and capping activities. Next, we characterized two new gamma TuRC subunits, Dgrips128 and 163, and showed that they are centrosomal proteins. Sequence comparisons among all known gamma TuRC subunits revealed two novel sequence motifs, which we named grip motifs 1 and 2. We found that Drips128 and 163 can each interact with gamma TuSC. However, this interaction is insufficient for gamma TuRC assembly.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/21 alle ore 02:40:05