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Titolo:
Metaphase to anaphase (mat) transition-defective Mutants in Caenorhabditiselegans
Autore:
Golden, A; Sadler, PL; Wallenfang, MR; Schumacher, JM; Hamill, DR; Bates, G; Bowerman, B; Seydoux, G; Shakes, DC;
Indirizzi:
Coll William & Mary, Dept Biol, Williamsburg, VA 23187 USA Coll William & Mary Williamsburg VA USA 23187 Williamsburg, VA 23187 USA NIDDKD, Lab Biochem & Genet, NIH, Bethesda, MD 20892 USA NIDDKD Bethesda MD USA 20892 Biochem & Genet, NIH, Bethesda, MD 20892 USA Univ Houston, Dept Biol, Houston, TX 77204 USA Univ Houston Houston TX USA 77204 uston, Dept Biol, Houston, TX 77204 USA Johns Hopkins Univ, Sch Med, Dept Mol Biol & Genet, Baltimore, MD 21205 USA Johns Hopkins Univ Baltimore MD USA 21205 Genet, Baltimore, MD 21205 USA Univ Texas, MD Anderson Canc Ctr, Dept Mol Genet, Houston, TX 77030 USA Univ Texas Houston TX USA 77030 tr, Dept Mol Genet, Houston, TX 77030 USA Univ Oregon, Inst Mol Biol, Eugene, OR 97403 USA Univ Oregon Eugene OR USA 97403 egon, Inst Mol Biol, Eugene, OR 97403 USA
Titolo Testata:
JOURNAL OF CELL BIOLOGY
fascicolo: 7, volume: 151, anno: 2000,
pagine: 1469 - 1482
SICI:
0021-9525(200012)151:7<1469:MTA(TM>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-PROTEIN INTERACTIONS; SENSITIVE-LETHAL MUTATIONS; SISTER-CHROMATID COHESION; C-ELEGANS; CHROMOSOME CONDENSATION; PROMOTING COMPLEX; HISTONE H3; GENETIC-ANALYSIS; CELL-DIVISION; MITOSIS;
Keywords:
meiosis; metaphase arrest; cell cycle; C. elegans; anaphase promoting complex;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
70
Recensione:
Indirizzi per estratti:
Indirizzo: Shakes, DC Coll William & Mary, Dept Biol, POB 8795, Williamsburg, VA 23187 USA Coll William & Mary POB 8795 Williamsburg VA USA 23187 3187 USA
Citazione:
A. Golden et al., "Metaphase to anaphase (mat) transition-defective Mutants in Caenorhabditiselegans", J CELL BIOL, 151(7), 2000, pp. 1469-1482

Abstract

The metaphase to anaphase transition is a critical stage of the eukaryoticcell cycle, and, thus, it is highly regulated. Errors during this transition can lead to chromosome segregation defects and death of the organism. Ingenetic screens for temperature-sensitive maternal effect embryonic lethal(Me1) mutants, we have identified 32 mutants in the nematode Caenorhabditis elegans in which fertilized embryos arrest as one-cell embryos. In these mutant embryos, the oocyte chromosomes arrest in metaphase of meiosis I without transitioning to anaphase or producing polar bodies. An additional block in M phase exit is evidenced by the failure to form pronuclei and the persistence of phosphohistone H3 and MPM-2 antibody staining. Spermatocyte meiosis is also perturbed; primary spermatocytes arrest in metaphase of meiosis I and fail to produce secondary spermatocytes. Analogous mitotic defectscause M phase delays in mitotic germline proliferation. We have named thisclass of mutants "mat" for metaphase to anaphase transition defective. These mutants, representing six different complementation groups, all map neargenes that encode subunits of the anaphase promoting complex or cyclosome,and, here, we show that one of the genes, emb-27, encodes the C. elegans CDC16 ortholog.

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Documento generato il 27/11/20 alle ore 22:00:30